| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2017-03-15 |
| タイトル |
|
|
タイトル |
Salmon DNA Accelerates Bone Regeneration by Inducing Osteoblast Migration. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
DNA |
| キーワード |
|
|
主題 |
骨再生 |
| キーワード |
|
|
主題 |
骨分化 |
| キーワード |
|
|
主題 |
頭蓋骨欠損 |
| キーワード |
|
|
主題 |
細胞遊走 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Sato, Ayako
Kajiya, Hiroshi
Mori, Nana
Sato, Hironobu
Fukushima, Tadao
Kido, Hirofumi
Ohno, Jun
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The initial step of bone regeneration requires the migration of osteogenic cells to defective sites. Our previous studies suggest that a salmon DNA-based scaffold can promote the bone regeneration of calvarial defects in rats. We speculate that the salmon DNA may possess osteoinductive properties, including the homing of migrating osteogenic cells. In the present study, we investigated the influence of the salmon DNA on osteoblastic differentiation and induction of osteoblast migration using MG63 cells (human preosteoblasts) in vitro. Moreover, we analyzed the bone regeneration of a critical-sized in vivo calvarial bone defect (CSD) model in rats. The salmon DNA enhanced both mRNA and protein expression of the osteogenesis-related factors, runt-related transcription factor 2 (Runx2), alkaline phosphatase, and osterix (OSX) in the MG63 cells, compared with the cultivation using osteogenic induction medium alone. From the histochemical and immunohistochemical assays using frozen sections of the bone defects from animals that were implanted with DNA disks, many cells were found to express aldehyde dehydrogenase 1, one of the markers for mesenchymal stem cells. In addition, OSX was observed in the replaced connective tissue of the bone defects. These findings indicate that the DNA induced the migration and accumulation of osteogenic cells to the regenerative tissue. Furthermore, an in vitro transwell migration assay showed that the addition of DNA enhanced an induction of osteoblast migration, compared with the medium alone. The implantation of the DNA disks promoted bone regeneration in the CSD of rats, compared with that of collagen disks. These results indicate that the salmon DNA enhanced osteoblastic differentiation and induction of migration, resulting in the facilitation of bone regeneration. |
| 学位名 |
|
|
学位名 |
博士（歯学） |
| 学位授与大学 |
|
|
|
学位授与機関識別子 |
37114 |
|
|
学位授与機関名 |
福岡歯科大学 |
| 学位授与年度 |
|
|
内容記述 |
2016年度 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2017-03-11 |
| 報告番号 |
|
|
学位授与番号 |
甲第287号 |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169522 |
|
|
関連名称 |
PLOS ONE |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
甲第287号_論文(佐藤) (2.1 MB)
