{"created":"2023-05-15T09:58:09.632347+00:00","id":69,"links":{},"metadata":{"_buckets":{"deposit":"774f3719-c409-44f8-802d-89706e384419"},"_deposit":{"created_by":15,"id":"69","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"69"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000069","sets":["1:14:15"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2017-03-11"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学 "}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2016年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The initial step of bone regeneration requires the migration of osteogenic cells to defective sites. Our previous studies suggest that a salmon DNA-based scaffold can promote the bone regeneration of calvarial defects in rats. We speculate that the salmon DNA may possess osteoinductive properties, including the homing of migrating osteogenic cells. In the present study, we investigated the influence of the salmon DNA on osteoblastic differentiation and induction of osteoblast migration using MG63 cells (human preosteoblasts) in vitro. Moreover, we analyzed the bone regeneration of a critical-sized in vivo calvarial bone defect (CSD) model in rats. The salmon DNA enhanced both mRNA and protein expression of the osteogenesis-related factors, runt-related transcription factor 2 (Runx2), alkaline phosphatase, and osterix (OSX) in the MG63 cells, compared with the cultivation using osteogenic induction medium alone. From the histochemical and immunohistochemical assays using frozen sections of the bone defects from animals that were implanted with DNA disks, many cells were found to express aldehyde dehydrogenase 1, one of the markers for mesenchymal stem cells. In addition, OSX was observed in the replaced connective tissue of the bone defects. These findings indicate that the DNA induced the migration and accumulation of osteogenic cells to the regenerative tissue. Furthermore, an in vitro transwell migration assay showed that the addition of DNA enhanced an induction of osteoblast migration, compared with the medium alone. The implantation of the DNA disks promoted bone regeneration in the CSD of rats, compared with that of collagen disks. These results indicate that the salmon DNA enhanced osteoblastic differentiation and induction of migration, resulting in the facilitation of bone regeneration.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第287号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"PLOS ONE"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169522","subitem_relation_type_select":"URI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sato, Ayako"}]},{"creatorNames":[{"creatorName":"Kajiya, Hiroshi"}]},{"creatorNames":[{"creatorName":"Mori, Nana"}]},{"creatorNames":[{"creatorName":"Sato, Hironobu"}]},{"creatorNames":[{"creatorName":"Fukushima, Tadao"}]},{"creatorNames":[{"creatorName":"Kido, Hirofumi"}]},{"creatorNames":[{"creatorName":"Ohno, Jun"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-15"}],"displaytype":"detail","filename":"甲第287号_論文(佐藤).pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"甲第287号_論文(佐藤).pdf","mimetype":"application/pdf","url":{"label":"甲第287号_論文(佐藤)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/69/files/甲第287号_論文(佐藤).pdf"},"version_id":"5a920673-42ca-47ea-b8a1-b3887ba50df5"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-15"}],"displaytype":"detail","filename":"甲第287号_論文内容の要旨(佐藤).pdf","filesize":[{"value":"148.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://fdc.repo.nii.ac.jp/record/69/files/甲第287号_論文内容の要旨(佐藤).pdf"},"version_id":"d100fa3a-427c-44b4-9d19-71194c7e5a80"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-15"}],"displaytype":"detail","filename":"甲第287号_論文審査結果の要旨(佐藤).pdf","filesize":[{"value":"208.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査結果の要旨","url":"https://fdc.repo.nii.ac.jp/record/69/files/甲第287号_論文審査結果の要旨(佐藤).pdf"},"version_id":"4eaae6cf-98f8-43a8-9591-124a23de6e8f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"DNA"},{"subitem_subject":"骨再生"},{"subitem_subject":"骨分化"},{"subitem_subject":"頭蓋骨欠損"},{"subitem_subject":"細胞遊走"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Salmon DNA Accelerates Bone Regeneration by Inducing Osteoblast Migration.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Salmon DNA Accelerates Bone Regeneration by Inducing Osteoblast Migration.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["15"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-03-15"},"publish_date":"2017-03-15","publish_status":"0","recid":"69","relation_version_is_last":true,"title":["Salmon DNA Accelerates Bone Regeneration by Inducing Osteoblast Migration."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-06-16T00:39:50.617548+00:00"}