| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2016-04-22 |
| タイトル |
|
|
タイトル |
Osteogenic potential for replacing cells in rat cranial defects implanted with a DNA/protamine complex paste. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題 |
Animals |
| キーワード |
|
|
言語 |
en |
|
主題 |
Bone Regeneration |
| キーワード |
|
|
言語 |
en |
|
主題 |
DNA |
| キーワード |
|
|
言語 |
en |
|
主題 |
Male |
| キーワード |
|
|
言語 |
en |
|
主題 |
Osteogenesis |
| キーワード |
|
|
言語 |
en |
|
主題 |
Protamines |
| キーワード |
|
|
言語 |
en |
|
主題 |
Rats |
| キーワード |
|
|
言語 |
en |
|
主題 |
Rats, Sprague-Dawley |
| キーワード |
|
|
言語 |
en |
|
主題 |
Skull |
| キーワード |
|
|
言語 |
en |
|
主題 |
depression |
| キーワード |
|
|
言語 |
en |
|
主題 |
insomnia |
| キーワード |
|
|
言語 |
en |
|
主題 |
old-old |
| キーワード |
|
|
言語 |
en |
|
主題 |
oral-health related quality of life |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Toda, Masako
Ohno, Jun
Shinozaki, Yosuke
Ozaki, Masao
Fukushima, Tadao
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Osteoinductive scaffolds are required for bone tissue engineering. The aim of the present study was to assess the osteoinductive capacity of deoxyribonucleic acid (DNA)/protamine complexes in a rat model of critical-size calvarial defects. In addition, we investigated whether cultured mesenchymal-like cells (DP-cells) outgrown from DNA/protamine complex engrafted defects could differentiate to become osteogenic cells in vitro. DNA/protamine complexes were prepared by reactions between DNA and protamine sulfate solutions with stirring. Critical-sized (8mm) calvarial defects were created in the central parietal bones of adult rats. Defects were either left empty or treated with DNA/protamine complex scaffolds. Subsequently, micro-computed tomography (micro-CT), histological, and immunohistochemical analyses were performed. Micro-CT and histological assays showed that DNA/protamine complex engrafted defects had enhanced bone regeneration. DP-cells were expanded from explants of DNA/protamine complex engrafted defects using an explant outgrowth culture system. Osteogenesis-related factors were assessed in DP-cells after treatment with an osteoblast-inducing reagent (OIR). After 3months, nearly complete healing was observed for DNA/protamine complex engrafted calvarial defects. Increased alkaline phosphatase (ALP) activity and Alizarin red staining were found for cultured DP-cells. These cells had high expression levels of osteogenic genes, including those for RUNX-2, ALP, osteopontin, and osteocalcin. These results indicated that DNA/protamine complexes could facilitate bone regeneration in calvarial defects. Moreover, in vitro osteogenic induction experiments showed that DP-cells outgrown from DNA/protamine engrafted defects had an osteogenic potential. Based on these results, we suggest that DNA/protamine complexes may recruit osteocompetent cells in these defects, where they differentiate to osteogenic cells. |
| 学位名 |
|
|
学位名 |
博士（歯学） |
| 学位授与大学 |
|
|
|
学位授与機関識別子 |
37114 |
|
|
学位授与機関名 |
福岡歯科大学 |
| 学位授与年度 |
|
|
内容記述 |
2015年度 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2016-03-12 |
| 報告番号 |
|
|
学位授与番号 |
甲第275号 |
| 情報源 |
|
|
|
関連名称 |
Bone. 2014 Oct;67:237-45. doi: 10.1016/j.bone.2014.07.018. Epub 2014 Jul 19. |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
http://www.sciencedirect.com/science/article/pii/S8756328214002701 |
|
|
関連名称 |
ScienceDirect |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
甲第275号_論文(戸田) (2.7 MB)
