{"created":"2023-05-15T09:58:09.154837+00:00","id":56,"links":{},"metadata":{"_buckets":{"deposit":"da0f8219-5179-4d29-9e74-3ac9d365f280"},"_deposit":{"created_by":15,"id":"56","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"56"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000056","sets":["1:11:12"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2016-03-12"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学） "}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2015年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Osteoinductive scaffolds are required for bone tissue engineering. The aim of the present study was to assess the osteoinductive capacity of deoxyribonucleic acid (DNA)/protamine complexes in a rat model of critical-size calvarial defects. In addition, we investigated whether cultured mesenchymal-like cells (DP-cells) outgrown from DNA/protamine complex engrafted defects could differentiate to become osteogenic cells in vitro. DNA/protamine complexes were prepared by reactions between DNA and protamine sulfate solutions with stirring. Critical-sized (8mm) calvarial defects were created in the central parietal bones of adult rats. Defects were either left empty or treated with DNA/protamine complex scaffolds. Subsequently, micro-computed tomography (micro-CT), histological, and immunohistochemical analyses were performed. Micro-CT and histological assays showed that DNA/protamine complex engrafted defects had enhanced bone regeneration. DP-cells were expanded from explants of DNA/protamine complex engrafted defects using an explant outgrowth culture system. Osteogenesis-related factors were assessed in DP-cells after treatment with an osteoblast-inducing reagent (OIR). After 3months, nearly complete healing was observed for DNA/protamine complex engrafted calvarial defects. Increased alkaline phosphatase (ALP) activity and Alizarin red staining were found for cultured DP-cells. These cells had high expression levels of osteogenic genes, including those for RUNX-2, ALP, osteopontin, and osteocalcin. These results indicated that DNA/protamine complexes could facilitate bone regeneration in calvarial defects. Moreover, in vitro osteogenic induction experiments showed that DP-cells outgrown from DNA/protamine engrafted defects had an osteogenic potential. Based on these results, we suggest that DNA/protamine complexes may recruit osteocompetent cells in these defects, where they differentiate to osteogenic cells.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第275号"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Bone. 2014 Oct;67:237-45. doi: 10.1016/j.bone.2014.07.018. Epub 2014 Jul 19."}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"ScienceDirect"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.sciencedirect.com/science/article/pii/S8756328214002701","subitem_relation_type_select":"URI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Toda, Masako"}]},{"creatorNames":[{"creatorName":"Ohno, Jun"}]},{"creatorNames":[{"creatorName":"Shinozaki, Yosuke"}]},{"creatorNames":[{"creatorName":"Ozaki, Masao"}]},{"creatorNames":[{"creatorName":"Fukushima, Tadao"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-22"}],"displaytype":"detail","filename":"甲第275号_論文(戸田).pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第275号_論文(戸田)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/56/files/甲第275号_論文(戸田).pdf"},"version_id":"4e66ed92-0c49-4270-bcdf-c188884529fc"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-22"}],"displaytype":"detail","filename":"甲第275号_論文内容の要旨(戸田).pdf","filesize":[{"value":"94.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://fdc.repo.nii.ac.jp/record/56/files/甲第275号_論文内容の要旨(戸田).pdf"},"version_id":"9757eaee-085c-4da5-a4ec-58e787e4ddaa"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-04-22"}],"displaytype":"detail","filename":"甲第275号_論文審査結果の要旨(戸田).pdf","filesize":[{"value":"110.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査結果の要旨","url":"https://fdc.repo.nii.ac.jp/record/56/files/甲第275号_論文審査結果の要旨(戸田).pdf"},"version_id":"2d7e6b23-49d6-4e0f-bfb6-dd846f08c3ff"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Animals","subitem_subject_language":"en"},{"subitem_subject":"Bone Regeneration","subitem_subject_language":"en"},{"subitem_subject":"DNA","subitem_subject_language":"en"},{"subitem_subject":"Male","subitem_subject_language":"en"},{"subitem_subject":"Osteogenesis","subitem_subject_language":"en"},{"subitem_subject":"Protamines","subitem_subject_language":"en"},{"subitem_subject":"Rats","subitem_subject_language":"en"},{"subitem_subject":"Rats, Sprague-Dawley","subitem_subject_language":"en"},{"subitem_subject":"Skull","subitem_subject_language":"en"},{"subitem_subject":"depression","subitem_subject_language":"en"},{"subitem_subject":"insomnia","subitem_subject_language":"en"},{"subitem_subject":"old-old","subitem_subject_language":"en"},{"subitem_subject":"oral-health related quality of life","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Osteogenic potential for replacing cells in rat cranial defects implanted with a DNA/protamine complex paste.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Osteogenic potential for replacing cells in rat cranial defects implanted with a DNA/protamine complex paste.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["12"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-04-22"},"publish_date":"2016-04-22","publish_status":"0","recid":"56","relation_version_is_last":true,"title":["Osteogenic potential for replacing cells in rat cranial defects implanted with a DNA/protamine complex paste."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-06-24T02:00:33.277380+00:00"}