| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2015-07-23 |
| タイトル |
|
|
タイトル |
Matrix metalloproteinase-2 degrades fibrillin-1 and fibrillin-2 of oxytalan fibers in the human eye and periodontal ligaments in vitro. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
fibrillin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
microfibrils |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
oxytalan fibers |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
ciliary zonule |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Kawagoe, Megumi
Tsuruga, Eichi
Oka, Kyoko
Sawa, Yoshihiko
Ishikawa, Hiroyuki
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Oxytalan fibers are distributed in the eye and periodontal ligaments (PDL). The ciliary zonule, known as Zinn's zonule, in the eye is composed of oxytalan fibers, which are bundles of microfibrils consisting mainly of fibrillin-1 and fibrillin-2. As turnover of oxytalan fibers is slow during life, their degradation mechanism remains unclarified. This study was performed to examine degradation pattern of fibrillin-1 and fibrillin-2 by experimental MMP activation. We cultured human non-pigmented ciliary epithelial cells (HNPCEC) and PDL fibroblasts for 7 days, then treated them with concanavalin A to activate matrix metalloproteinase (MMP)-2, and examined the degradation of fibrillin-1 and fibrillin-2 for 72 hr using immunofluorescence. At 7 days of HNPCEC culture, fibrillin-1-positive fibers were observed, some of which merged with fibrillin-2. After MMP-2 activation, fibrillin-1-positive fibers became thin and disappeared by 72 hr, while fibrillin-2-positive fibers disappeared almost completely within 24 hr. At 7 days of PDL fibroblast culture, fibrillin-1-positive fibers were mostly merged with fibrillin-2. After MMP-2 activation, fibrillin-1-positive fibers became thin by 24 hr and had almost disappeared by 48 hr, while fibrillin-2-positive fibers decreased constantly after 24 hr. A MMP-2 inhibitor completely suppressed these degradations. These results suggest that the patterns of fibrillin-1 and fibrillin-2 degradation differ between the eye and the PDL, possibly reflecting the sensitivity of fibrillin-1 and fibrillin-2 of each type of oxytalan fiber against MMP-2. |
| 学位名 |
|
|
学位名 |
博士（歯学） |
| 学位授与大学 |
|
|
|
学位授与機関識別子Scheme |
kakenhi |
|
|
学位授与機関識別子 |
37114 |
|
|
学位授与機関名 |
福岡歯科大学 |
| 学位授与年度 |
|
|
内容記述 |
2014年度 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2015-03-14 |
| 報告番号 |
|
|
学位授与番号 |
甲第258号 |
| 情報源 |
|
|
|
関連名称 |
Acta Histochem Cytochem. 2013 Oct 30;46(5):153-9. doi: 10.1267/ahc.13024. Epub 2013 Oct 23. |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://www.jstage.jst.go.jp/article/ahc/46/5/46_13024/_article |
|
|
関連名称 |
J-STAGE |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
甲第258号_論文(川越) (776.1 kB)
