{"created":"2023-05-15T09:58:08.890936+00:00","id":49,"links":{},"metadata":{"_buckets":{"deposit":"846565d1-cd7c-4070-b792-55ce64e6578f"},"_deposit":{"created_by":15,"id":"49","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"49"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000049","sets":["1:7:8"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-03-14"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2014年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Oxytalan fibers are distributed in the eye and periodontal ligaments (PDL). The ciliary zonule, known as Zinn's zonule, in the eye is composed of oxytalan fibers, which are bundles of microfibrils consisting mainly of fibrillin-1 and fibrillin-2. As turnover of oxytalan fibers is slow during life, their degradation mechanism remains unclarified. This study was performed to examine degradation pattern of fibrillin-1 and fibrillin-2 by experimental MMP activation. We cultured human non-pigmented ciliary epithelial cells (HNPCEC) and PDL fibroblasts for 7 days, then treated them with concanavalin A to activate matrix metalloproteinase (MMP)-2, and examined the degradation of fibrillin-1 and fibrillin-2 for 72 hr using immunofluorescence. At 7 days of HNPCEC culture, fibrillin-1-positive fibers were observed, some of which merged with fibrillin-2. After MMP-2 activation, fibrillin-1-positive fibers became thin and disappeared by 72 hr, while fibrillin-2-positive fibers disappeared almost completely within 24 hr. At 7 days of PDL fibroblast culture, fibrillin-1-positive fibers were mostly merged with fibrillin-2. After MMP-2 activation, fibrillin-1-positive fibers became thin by 24 hr and had almost disappeared by 48 hr, while fibrillin-2-positive fibers decreased constantly after 24 hr. A MMP-2 inhibitor completely suppressed these degradations. These results suggest that the patterns of fibrillin-1 and fibrillin-2 degradation differ between the eye and the PDL, possibly reflecting the sensitivity of fibrillin-1 and fibrillin-2 of each type of oxytalan fiber against MMP-2.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第258号"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Acta Histochem Cytochem. 2013 Oct 30;46(5):153-9. doi: 10.1267/ahc.13024. Epub 2013 Oct 23."}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"J-STAGE"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/article/ahc/46/5/46_13024/_article","subitem_relation_type_select":"URI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawagoe, Megumi"}]},{"creatorNames":[{"creatorName":"Tsuruga, Eichi"}]},{"creatorNames":[{"creatorName":"Oka, Kyoko"}]},{"creatorNames":[{"creatorName":"Sawa, Yoshihiko"}]},{"creatorNames":[{"creatorName":"Ishikawa, Hiroyuki"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-07-23"}],"displaytype":"detail","filename":"甲第258号_論文(川越).pdf","filesize":[{"value":"776.1 kB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第258号_論文(川越)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/49/files/甲第258号_論文(川越).pdf"},"version_id":"3560fb18-e160-4458-b279-c7afdfdd86b4"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-07-23"}],"displaytype":"detail","filename":"甲第258号_論文内容の要旨(川越).pdf","filesize":[{"value":"424.0 kB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://fdc.repo.nii.ac.jp/record/49/files/甲第258号_論文内容の要旨(川越).pdf"},"version_id":"5cf13b00-b15e-4f5d-8b63-c39043b19be7"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-07-23"}],"displaytype":"detail","filename":"甲第258号_論文審査結果の要旨(川越).pdf","filesize":[{"value":"242.6 kB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査結果の要旨","url":"https://fdc.repo.nii.ac.jp/record/49/files/甲第258号_論文審査結果の要旨(川越).pdf"},"version_id":"27024544-59ed-4607-9a79-f545e61a30cf"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"fibrillin","subitem_subject_language":"en"},{"subitem_subject":"microfibrils","subitem_subject_language":"en"},{"subitem_subject":"oxytalan fibers","subitem_subject_language":"en"},{"subitem_subject":"ciliary zonule","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Matrix metalloproteinase-2 degrades fibrillin-1 and fibrillin-2 of oxytalan fibers in the human eye and periodontal ligaments in vitro.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Matrix metalloproteinase-2 degrades fibrillin-1 and fibrillin-2 of oxytalan fibers in the human eye and periodontal ligaments in vitro.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["8"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-07-23"},"publish_date":"2015-07-23","publish_status":"0","recid":"49","relation_version_is_last":true,"title":["Matrix metalloproteinase-2 degrades fibrillin-1 and fibrillin-2 of oxytalan fibers in the human eye and periodontal ligaments in vitro."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-11T01:22:23.492252+00:00"}