| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2015-03-27 |
| タイトル |
|
|
タイトル |
Photothermal stress triggered by near infrared-irradiated carbon nanotubes promotes bone deposition in rat calvarial defects. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
温熱刺激 |
| キーワード |
|
|
主題 |
カーボンナノチューブ |
| キーワード |
|
|
主題 |
近赤外線 |
| キーワード |
|
|
主題 |
DNA/プロタミン複合体 |
| キーワード |
|
|
主題 |
骨形成 |
| キーワード |
|
|
言語 |
en |
|
主題 |
Photothermal stress |
| キーワード |
|
|
言語 |
en |
|
主題 |
Carbon nano tubes |
| キーワード |
|
|
言語 |
en |
|
主題 |
Near infrared ray |
| キーワード |
|
|
言語 |
en |
|
主題 |
DNA/Protamine complex |
| キーワード |
|
|
言語 |
en |
|
主題 |
Osteogenesis |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Yanagi, Tsukasa
Kajiya, Hiroshi
Kawaguchi, Minoru
Kido, Hirofumi
Fukushima, Tadao
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The bone regenerative healing process is often prolonged, with a high risk of infection particularly in elderly and diseased patients. A reduction in healing process time usually requires mechanical stress devices, chemical cues, or laser/thermal therapies. Although these approaches have been used extensively for the reduction of bone healing time, the exact mechanisms involved in thermal stress-induced bone regeneration remain unclear. In this study, we investigated the effect of optimal hyperthermia on rat calvarial defects in vivo and on osteogenesis in vitro. Photothermal stress stimulation was carried out using a new photothermal device, composed of an alginate gel including in carbon nanotubes and their irradiator with near-infrared light. Photothermal stress (15 min at 42℃, every day), trigged by near-infrared-induced carbon nanotube, promoted bone deposition in critical-sized calvarial defects compared with nonthermal stress controls. We recently reported that our novel DNA/protamine complex scaffold induces bone regeneration in calvarial defects. In this study, photothermal stress upregulated bone deposition in DNA/protamine-engrafted calvarial defects. Furthermore, photothermal stress significantly induced expression of osteogenic related genes in a time-dependent manner, including alkaline phosphatase, osterix, and osteocalcin. This was observed in DNA/protamine cells, which were expanded from regenerated tissue engrafted into the DNA/protamine scaffold, as well as in human MG63 preosteoblasts. In summary, this novel carbon nanotube-based photothermal stress approach upregulated expression of osteogenic-related genes in preosteoblasts, resulting in promotion of mineral deposition for enhanced bone repair. |
| 学位名 |
|
|
学位名 |
博士（歯学） |
| 学位授与大学 |
|
|
|
学位授与機関識別子 |
37114 |
|
|
学位授与機関名 |
福岡歯科大学 |
| 学位授与年度 |
|
|
内容記述 |
2014年度 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2015-03-14 |
| 報告番号 |
|
|
学位授与番号 |
甲第260号 |
| 情報源 |
|
|
|
関連名称 |
J Biomater Appl. 2015 Mar;29(8):1109-18. doi: 10.1177/0885328214556913. Epub 2014 Oct 20. |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
甲第260号_論文(柳) (2.1 MB)
