| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2022-05-31 |
| タイトル |
|
|
タイトル |
Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Retinoic acid |
| キーワード |
|
|
主題 |
Retinoid X receptor |
| キーワード |
|
|
主題 |
Keratinocyte |
| キーワード |
|
|
主題 |
Three-dimensional culture |
| キーワード |
|
|
主題 |
Desmosome |
| キーワード |
|
|
主題 |
Tight junction |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Ishikawa, Shoko
Nikaido, Misaki
Otani, Takahito
Ogata, Kayoko
Iida, Hiroshi
Inai, Yuko
Tamaoki, Sachio
Inai, Tetsuichiro
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Retinoic acid (RA) plays an important role in epithelial homeostasis and influences the morphology, proliferation, differentiation and permeability of epithelial cells. Mouse keratinocytes, K38, reconstituted non-keratinized stratified epithelium in three-dimensional (3D) cultures with serum, which contains retinol (a source of RA), but the morphology was different from in vivo epithelium. The formed epithelium was thick, with loosened cell-cell contacts. Here, we investigated whether the inhibition of RA receptor (RAR)/retinoid X receptor (RXR)-mediated signaling by an RXR antagonist, HX 531, improved K38 3D cultures in terms of morphology and intercellular junctions. The epithelium formed by 0.5 μM HX531 was thin, and the intercellular space was narrowed because of the restoration of the layer-specific distribution of desmoglein (DSG)-1, DSG3 and plakoglobin (PG). Moreover, the levels of desmosomal proteins and tight junction proteins, including DSG1, DSG2, DSG3, PG, claudin (CLDN)-1 and CLDN4 increased, but the adherens junction protein, E-cadherin, did not show any change. Furthermore, CLDN1 was recruited to occludin-positive cell-cell contacts in the superficial cells and transepithelial electrical resistance was increased. Therefore, K38 3D cultures treated with 0.5 μM HX531 provides a useful in vitro model to study intercellular junctions in the non-keratinized epithelium. |
| 学位名 |
|
|
学位名 |
博士（歯学） |
| 学位授与大学 |
|
|
|
学位授与機関識別子 |
37114 |
|
|
学位授与機関名 |
福岡歯科大学 |
| 学位授与年度 |
|
|
内容記述 |
2021年度 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2022-03-31 |
| 報告番号 |
|
|
学位授与番号 |
甲第339号 |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://academic.oup.com/jmicro/advance-article/doi/10.1093/jmicro/dfac007/6530229?login=true |
|
|
関連名称 |
OXFORD |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
甲第339号_論文(石川翔子) (5.6 MB)
