{"created":"2023-05-15T09:58:12.417528+00:00","id":148,"links":{},"metadata":{"_buckets":{"deposit":"33bf8c22-4f51-4ed0-a9d5-bea0e04837e3"},"_deposit":{"created_by":15,"id":"148","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"148"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000148","sets":["1:29:30"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-03-31"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2021年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Retinoic acid (RA) plays an important role in epithelial homeostasis and influences the morphology, proliferation, differentiation and permeability of epithelial cells. Mouse keratinocytes, K38, reconstituted non-keratinized stratified epithelium in three-dimensional (3D) cultures with serum, which contains retinol (a source of RA), but the morphology was different from in vivo epithelium. The formed epithelium was thick, with loosened cell-cell contacts. Here, we investigated whether the inhibition of RA receptor (RAR)/retinoid X receptor (RXR)-mediated signaling by an RXR antagonist, HX 531, improved K38 3D cultures in terms of morphology and intercellular junctions. The epithelium formed by 0.5 μM HX531 was thin, and the intercellular space was narrowed because of the restoration of the layer-specific distribution of desmoglein (DSG)-1, DSG3 and plakoglobin (PG). Moreover, the levels of desmosomal proteins and tight junction proteins, including DSG1, DSG2, DSG3, PG, claudin (CLDN)-1 and CLDN4 increased, but the adherens junction protein, E-cadherin, did not show any change. Furthermore, CLDN1 was recruited to occludin-positive cell-cell contacts in the superficial cells and transepithelial electrical resistance was increased. Therefore, K38 3D cultures treated with 0.5 μM HX531 provides a useful in vitro model to study intercellular junctions in the non-keratinized epithelium.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第339号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"OXFORD"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://academic.oup.com/jmicro/advance-article/doi/10.1093/jmicro/dfac007/6530229?login=true","subitem_relation_type_select":"URI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ishikawa, Shoko"}]},{"creatorNames":[{"creatorName":"Nikaido, Misaki"}]},{"creatorNames":[{"creatorName":"Otani, Takahito"}]},{"creatorNames":[{"creatorName":"Ogata, Kayoko"}]},{"creatorNames":[{"creatorName":"Iida, Hiroshi"}]},{"creatorNames":[{"creatorName":"Inai, Yuko"}]},{"creatorNames":[{"creatorName":"Tamaoki, Sachio"}]},{"creatorNames":[{"creatorName":"Inai, Tetsuichiro"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-01"}],"displaytype":"detail","filename":"甲第339号_論文(石川翔子).pdf","filesize":[{"value":"5.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第339号_論文(石川翔子)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/148/files/甲第339号_論文(石川翔子).pdf"},"version_id":"13d9c304-e917-433b-856a-79091b9a1152"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-31"}],"displaytype":"detail","filename":"甲第339号_論文内容の要旨(石川翔子).pdf","filesize":[{"value":"197.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第339号_論文内容の要旨(石川翔子)","url":"https://fdc.repo.nii.ac.jp/record/148/files/甲第339号_論文内容の要旨(石川翔子).pdf"},"version_id":"d9dfc7a0-505c-4172-a0a2-53d839ff2739"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-31"}],"displaytype":"detail","filename":"甲第339号_論文審査結果の要旨(石川翔子).pdf","filesize":[{"value":"244.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第339号_論文審査結果の要旨(石川翔子)","url":"https://fdc.repo.nii.ac.jp/record/148/files/甲第339号_論文審査結果の要旨(石川翔子).pdf"},"version_id":"e654a908-6c31-44a4-ae79-9fdfab5b9f36"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Retinoic acid"},{"subitem_subject":"Retinoid X receptor"},{"subitem_subject":"Keratinocyte"},{"subitem_subject":"Three-dimensional culture"},{"subitem_subject":"Desmosome"},{"subitem_subject":"Tight junction"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["30"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-05-31"},"publish_date":"2022-05-31","publish_status":"0","recid":"148","relation_version_is_last":true,"title":["Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-11T01:14:48.091123+00:00"}