| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2019-04-11 |
| タイトル |
|
|
タイトル |
Peptidylarginine deiminase is involved in maintaining the cornified oral mucosa of rats. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Animals |
| キーワード |
|
|
主題 |
Animals, Newborn |
| キーワード |
|
|
主題 |
Blotting, Western |
| キーワード |
|
|
主題 |
Citrullination |
| キーワード |
|
|
主題 |
Electrophoresis, Gel, Two-Dimensional |
| キーワード |
|
|
主題 |
Fluorescent Antibody Technique |
| キーワード |
|
|
主題 |
Intermediate Filament Proteins |
| キーワード |
|
|
主題 |
Mouth Mucosa |
| キーワード |
|
|
主題 |
Protein-Arginine Deiminases |
| キーワード |
|
|
主題 |
RNA, Messenger |
| キーワード |
|
|
主題 |
Rats |
| キーワード |
|
|
主題 |
Rats, Wistar |
| キーワード |
|
|
主題 |
Real-Time Polymerase Chain Reaction |
| キーワード |
|
|
言語 |
en |
|
主題 |
Animals |
| キーワード |
|
|
言語 |
en |
|
主題 |
Animals, Newborn |
| キーワード |
|
|
言語 |
en |
|
主題 |
Blotting, Western |
| キーワード |
|
|
言語 |
en |
|
主題 |
Citrullination |
| キーワード |
|
|
言語 |
en |
|
主題 |
Electrophoresis, Gel, Two-Dimensional |
| キーワード |
|
|
言語 |
en |
|
主題 |
Fluorescent Antibody Technique |
| キーワード |
|
|
言語 |
en |
|
主題 |
Intermediate Filament Proteins |
| キーワード |
|
|
言語 |
en |
|
主題 |
Mouth Mucosa |
| キーワード |
|
|
言語 |
en |
|
主題 |
Protein-Arginine Deiminases |
| キーワード |
|
|
言語 |
en |
|
主題 |
RNA, Messenger |
| キーワード |
|
|
言語 |
en |
|
主題 |
Rats |
| キーワード |
|
|
言語 |
en |
|
主題 |
Rats, Wistar |
| キーワード |
|
|
言語 |
en |
|
主題 |
Real-Time Polymerase Chain Reaction |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Arita, Seiichi
Hatta, Mitsutoki
Uchida, Kunitoshi
Kita, Tomo
Okamura, Kazuhiko
Ryu, Takanori
Murakami, Hiroshi
Sakagami, Ryuji
Yamazaki, Jun
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Epithelial cells derived from different regions exhibit marked differences in their differentiation capacity, allowing them to provide a suitable protective barrier. We aimed to clarify the role of peptidylarginine deiminase (PAD) in modifying the key epidermal proteins filaggrin (FLG) and keratin 1 (K1) during stratification of the rat palate and buccal mucosa. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We performed immunofluorescence, immunoblotting, PAD activity assays and 2-dimensional electrophoresis, and developed an organotypic culture model. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
PAD1 expression was highest in the palate, whereas PAD2, PAD3 and PAD4 expression was highest in the skin, suggesting the tissue-specific expression of PAD isozymes that leads to differences in calcium dependency. Immunoblotting showed that the FLG monomer, as well as its degradation products and precursor (proFLG), were most abundantly expressed in the skin but had low expression in the palate, whereas only faint proFLG expression was detected in the buccal mucosa. FLG and K1 were colocalized with PAD1 and were likely to be citrullinated in the cornified layers of the skin; this colocalization was not detected on the palatal surface, and dot-like presence of proFLG that might be citrullinated and that of PAD1 were found in the granules of the palate. Organotypic models derived from the rat palate revealed that PAD inhibition reduced the breakdown of FLG, increased its association with K1 together with epithelial compaction, and decreased permeability in a dye permeability assay. Conversely, PAD stimulation had the opposite effects. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Citrullination is likely a protein modification that plays an important role in maintaining the structure and function of oral cornified mucosa in a way that is distinctly different from that of the skin. |
| 学位名 |
|
|
学位名 |
博士（歯学） |
| 学位授与大学 |
|
|
|
学位授与機関識別子 |
37114 |
|
|
学位授与機関名 |
福岡歯科大学 |
| 学位授与年度 |
|
|
内容記述 |
2018年度 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2018-04-27 |
| 報告番号 |
|
|
学位授与番号 |
甲第300号 |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://onlinelibrary.wiley.com/doi/full/10.1111/jre.12561 |
|
|
関連名称 |
WileyOnlineLibrary |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
甲第300号_論文(有田晴一) (1.1 MB)
