{"created":"2023-05-15T09:58:10.736029+00:00","id":102,"links":{},"metadata":{"_buckets":{"deposit":"9a32e3ea-b922-43f6-86af-0756f54855e6"},"_deposit":{"created_by":15,"id":"102","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"102"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000102","sets":["1:20:21"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-04-27"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2018年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Epithelial cells derived from different regions exhibit marked differences in their differentiation capacity, allowing them to provide a suitable protective barrier. We aimed to clarify the role of peptidylarginine deiminase (PAD) in modifying the key epidermal proteins filaggrin (FLG) and keratin 1 (K1) during stratification of the rat palate and buccal mucosa.","subitem_description_type":"Abstract"},{"subitem_description":"We performed immunofluorescence, immunoblotting, PAD activity assays and 2-dimensional electrophoresis, and developed an organotypic culture model.","subitem_description_type":"Abstract"},{"subitem_description":"PAD1 expression was highest in the palate, whereas PAD2, PAD3 and PAD4 expression was highest in the skin, suggesting the tissue-specific expression of PAD isozymes that leads to differences in calcium dependency. Immunoblotting showed that the FLG monomer, as well as its degradation products and precursor (proFLG), were most abundantly expressed in the skin but had low expression in the palate, whereas only faint proFLG expression was detected in the buccal mucosa. FLG and K1 were colocalized with PAD1 and were likely to be citrullinated in the cornified layers of the skin; this colocalization was not detected on the palatal surface, and dot-like presence of proFLG that might be citrullinated and that of PAD1 were found in the granules of the palate. Organotypic models derived from the rat palate revealed that PAD inhibition reduced the breakdown of FLG, increased its association with K1 together with epithelial compaction, and decreased permeability in a dye permeability assay. Conversely, PAD stimulation had the opposite effects.","subitem_description_type":"Abstract"},{"subitem_description":"Citrullination is likely a protein modification that plays an important role in maintaining the structure and function of oral cornified mucosa in a way that is distinctly different from that of the skin.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第300号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"WileyOnlineLibrary"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://onlinelibrary.wiley.com/doi/full/10.1111/jre.12561","subitem_relation_type_select":"URI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Arita, Seiichi"}]},{"creatorNames":[{"creatorName":"Hatta, Mitsutoki"}]},{"creatorNames":[{"creatorName":"Uchida, Kunitoshi"}]},{"creatorNames":[{"creatorName":"Kita, Tomo"}]},{"creatorNames":[{"creatorName":"Okamura, Kazuhiko"}]},{"creatorNames":[{"creatorName":"Ryu, Takanori"}]},{"creatorNames":[{"creatorName":"Murakami, Hiroshi"}]},{"creatorNames":[{"creatorName":"Sakagami, Ryuji"}]},{"creatorNames":[{"creatorName":"Yamazaki, Jun"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-23"}],"displaytype":"detail","filename":"甲第300号_論文(有田晴一).pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第300号_論文(有田晴一)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/102/files/甲第300号_論文(有田晴一).pdf"},"version_id":"eed20e1c-a52d-4f34-a52f-7a2745b8b917"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-11"}],"displaytype":"detail","filename":"甲第300号_論文内容の要旨(有田晴一).pdf","filesize":[{"value":"142.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第300号_論文内容の要旨(有田晴一)","url":"https://fdc.repo.nii.ac.jp/record/102/files/甲第300号_論文内容の要旨(有田晴一).pdf"},"version_id":"7c60be2b-25df-4818-a5e2-206a839cf51b"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-04-11"}],"displaytype":"detail","filename":"甲第300号_論文審査結果の要旨(有田晴一).pdf","filesize":[{"value":"202.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第300号_論文審査結果の要旨(有田晴一)","url":"https://fdc.repo.nii.ac.jp/record/102/files/甲第300号_論文審査結果の要旨(有田晴一).pdf"},"version_id":"6dd21798-be6f-49d3-a81a-fab975b4acfc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Animals"},{"subitem_subject":"Animals, Newborn"},{"subitem_subject":"Blotting, Western"},{"subitem_subject":"Citrullination"},{"subitem_subject":"Electrophoresis, Gel, Two-Dimensional"},{"subitem_subject":"Fluorescent Antibody Technique"},{"subitem_subject":"Intermediate Filament Proteins"},{"subitem_subject":"Mouth Mucosa"},{"subitem_subject":"Protein-Arginine Deiminases"},{"subitem_subject":"RNA, Messenger"},{"subitem_subject":"Rats"},{"subitem_subject":"Rats, Wistar"},{"subitem_subject":"Real-Time Polymerase Chain Reaction"},{"subitem_subject":"Animals","subitem_subject_language":"en"},{"subitem_subject":"Animals, Newborn","subitem_subject_language":"en"},{"subitem_subject":"Blotting, Western","subitem_subject_language":"en"},{"subitem_subject":"Citrullination","subitem_subject_language":"en"},{"subitem_subject":"Electrophoresis, Gel, Two-Dimensional","subitem_subject_language":"en"},{"subitem_subject":"Fluorescent Antibody Technique","subitem_subject_language":"en"},{"subitem_subject":"Intermediate Filament Proteins","subitem_subject_language":"en"},{"subitem_subject":"Mouth Mucosa","subitem_subject_language":"en"},{"subitem_subject":"Protein-Arginine Deiminases","subitem_subject_language":"en"},{"subitem_subject":"RNA, Messenger","subitem_subject_language":"en"},{"subitem_subject":"Rats","subitem_subject_language":"en"},{"subitem_subject":"Rats, Wistar","subitem_subject_language":"en"},{"subitem_subject":"Real-Time Polymerase Chain Reaction","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Peptidylarginine deiminase is involved in maintaining the cornified oral mucosa of rats.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Peptidylarginine deiminase is involved in maintaining the cornified oral mucosa of rats.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["21"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-04-11"},"publish_date":"2019-04-11","publish_status":"0","recid":"102","relation_version_is_last":true,"title":["Peptidylarginine deiminase is involved in maintaining the cornified oral mucosa of rats."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-06-25T22:32:58.566543+00:00"}