| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2017-08-01 |
| タイトル |
|
|
タイトル |
Formation of keratinocyte multilayers on filters under airlifted or submerged culture conditions in medium containing calcium, ascorbic acid, and keratinocyte growth factor. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Adult |
| キーワード |
|
|
主題 |
Ascorbic Acid |
| キーワード |
|
|
主題 |
Calcium |
| キーワード |
|
|
主題 |
Cells, Cultured |
| キーワード |
|
|
主題 |
Culture Media |
| キーワード |
|
|
主題 |
Fibroblast Growth Factor 7 |
| キーワード |
|
|
主題 |
Humans |
| キーワード |
|
|
主題 |
Keratinocytes |
| キーワード |
|
|
主題 |
Cytokeratins |
| キーワード |
|
|
主題 |
Integrin β4 |
| キーワード |
|
|
主題 |
Involucrin |
| キーワード |
|
|
主題 |
Organotypic culture |
| キーワード |
|
|
言語 |
en |
|
主題 |
Adult |
| キーワード |
|
|
言語 |
en |
|
主題 |
Ascorbic Acid |
| キーワード |
|
|
言語 |
en |
|
主題 |
Calcium |
| キーワード |
|
|
言語 |
en |
|
主題 |
Cells, Cultured |
| キーワード |
|
|
言語 |
en |
|
主題 |
Culture Media |
| キーワード |
|
|
言語 |
en |
|
主題 |
Fibroblast Growth Factor 7 |
| キーワード |
|
|
言語 |
en |
|
主題 |
Humans |
| キーワード |
|
|
言語 |
en |
|
主題 |
Keratinocytes |
| キーワード |
|
|
言語 |
en |
|
主題 |
Cytokeratins |
| キーワード |
|
|
言語 |
en |
|
主題 |
Integrin β4 |
| キーワード |
|
|
言語 |
en |
|
主題 |
Involucrin |
| キーワード |
|
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題 |
Organotypic culture |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Seo, Akira
Kitagawa, Norio
Matsuura, Takashi
Sato, Hironobu
Inai, Tetsuichiro
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Three-dimensional (3D) cell culture is a powerful in vitro technique to study the stratification and differentiation of keratinocytes. However, culture conditions, including culture media, supplements, and scaffolds (e.g., collagen gels with or without fibroblasts), can vary considerably. Here, we evaluated the roles of calcium, L-ascorbic acid phosphate magnesium salt n-hydrate (APM), and keratinocyte growth factor (KGF) in a chemically defined medium, EpiLife, in 3D cultures of primary human epidermal keratinocytes directly plated on polycarbonate filter inserts under airlifted or submerged conditions. Eight culture media containing various combinations of these three supplements were examined. Calcium was necessary for the stratification and differentiation of keratinocytes based on the localization of keratins and involucrin. However, the localization patterns of keratins and integrin β4 were partially disrupted and Ki67-positive basal cells almost disappeared 3 weeks after airlift. The addition of KGF, but not APM, prevented these changes. Further addition of APM markedly improved the tissue architecture, including basal cell morphology and the appearance of keratohyalin granules and localized involucrin in the upper suprabasal cells, even after 1 week. Although the submerged culture also formed cornified epithelium-like multilayers, involucrin was localized in the cornified layer, where nuclei were often found. Based on these results, it is most effective to culture keratinocytes at the air-liquid interface in EpiLife medium supplemented with calcium, APM, and KGF to form well-organized and orthokeratinized multilayers as skin analogues. |
| 学位名 |
|
|
学位名 |
博士（歯学） |
| 学位授与大学 |
|
|
|
学位授与機関識別子 |
37114 |
|
|
学位授与機関名 |
福岡歯科大学 |
| 学位授与年度 |
|
|
内容記述 |
2016年度 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2017-03-11 |
| 報告番号 |
|
|
学位授与番号 |
甲第283号 |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://link.springer.com/article/10.1007%2Fs00418-016-1472-1 |
|
|
関連名称 |
SpringerLink |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
甲第283号_論文(瀬尾) (1.4 MB)
