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Immunohistochemical expression of fibrillin-1 and fibrillin-2 during tooth development.
https://fdc.repo.nii.ac.jp/records/46
https://fdc.repo.nii.ac.jp/records/464cf409f9-79c4-46c2-ba96-b33158fb26bf
| 名前 / ファイル | ライセンス | アクション |
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甲第269号_論文(立岡) (4.4 MB)
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要旨 (399.9 kB)
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審査結果の要旨 (220.8 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||||||
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| 公開日 | 2015-07-07 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Immunohistochemical expression of fibrillin-1 and fibrillin-2 during tooth development. | |||||||||||||||
| 言語 | en | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題 | fibrillin | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題 | Hertwig's epithelial sheath | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題 | Oxytalan fiber | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題 | Periodontal ligament | |||||||||||||||
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| 言語 | en | |||||||||||||||
| 主題 | fibrillin | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題 | Hertwig's epithelial sheath | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題 | Oxytalan fiber | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題 | Periodontal ligament | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||||||
| 資源タイプ | doctoral thesis | |||||||||||||||
| アクセス権 | ||||||||||||||||
| アクセス権 | open access | |||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||
| 著者 |
Kira-Tatsuoka, Michiko
× Kira-Tatsuoka, Michiko
× Oka, Kyoko
× Tsuruga, Eichi
× Ozaki, Masao
× Sawa, Yoshihiko
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| 抄録 | ||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||
| 内容記述 | BACKGROUND AND OBJECTIVE: Oxytalan fibers are categorized as a microfibril assembly without elastin deposition, and are unique components in the periodontal ligament (PDL). However, little is known about their formation during PDL development. To clarify the mechanisms of oxytalan fiber formation in developing PDL, we performed immunohistochemical analysis to detect the direct expression of fibrillin-1 and fibrillin-2, which are major components of microfibrils. MATERIAL AND METHODS: Frozen sections of lower molars from mice at several stages of growth were prepared without chemical fixation and decalcification using the film transfer method. Immunostaining was performed with anti-fibrillin-1 and -2, and anticytokeratin antibodies. RESULTS: Fibrillin-1 was not expressed in the dental follicle during the crown forming stage. At postneonatal day 9, fibrillin-1 expression started with meshwork appearance between the epithelial cells from Hertwig's epithelial root sheath at the root dentin surface. Fibirillin-2 was detected much earlier than fibrillin-1 expression. Fibrillin-2 was expressed with a liner appearance, running parallel to the root axis in PDL, and was partially co-expressed with cytokeratin 14 expression in Hertwig's epithelial root sheath. Furthermore, we detected both fibrillin-1 and fibrillin-2 expression in human PDL. Fibrillin-1 was detected in fibers with a vertically oriented root axis in PDL. Fibrillin-2 was widely expressed in PDL, including around the epithelial cell rests of Malassez. Fibrillin-1 and fibrillin-2 were clearly co-expressed in thick fiber structures in human PDL. CONCLUSION: Our results suggest that both fibrillin-1 and fibrillin-2 expression is required to form thick oxytalan fibers in PDL. Based on the expression patterns for fibrillin-1 and fibrillin-2, they have different functions during tooth root and PDL development. Early expression of fibrillin-2 may regulate dental epithelial cell behavior during root and PDL development. |
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| 学位名 | ||||||||||||||||
| 学位名 | 博士（歯学） | |||||||||||||||
| 学位授与大学 | ||||||||||||||||
| 学位授与機関識別子 | 37114 | |||||||||||||||
| 学位授与機関名 | 福岡歯科大学 | |||||||||||||||
| 学位授与年度 | ||||||||||||||||
| 内容記述 | 2014年度 | |||||||||||||||
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| 学位授与年月日 | 2015-03-14 | |||||||||||||||
| 報告番号 | ||||||||||||||||
| 学位授与番号 | 甲第269号 | |||||||||||||||
| 情報源 | ||||||||||||||||
| 関連名称 | J Periodontal Res. 2014 Dec 18. doi: 10.1111/jre.12256. | |||||||||||||||
| 著者版フラグ | ||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||
