| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2014-07-15 |
| タイトル |
|
|
タイトル |
Association of caspase-14 and filaggrin expression with keratinization of the oral mucosa and reconstruction culture rat models. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題 |
caspase-14 |
| キーワード |
|
|
言語 |
en |
|
主題 |
filaggrin |
| キーワード |
|
|
言語 |
en |
|
主題 |
keratinization |
| キーワード |
|
|
言語 |
en |
|
主題 |
oral mucosa |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Murakami, Hiroshi
Okamura, Kazuhiko
Aoki, Shigehisa
Sakagami, Ryuji
Yamazaki, Jun
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Keratinization of the oral mucosa, such as the gingiva, has been shown to be important for periodontal health. Caspase-14 is a protease that plays a role in keratinization of the epidermis. The objective of this study was to investigate whether the expression of caspase-14 is intimately linked with keratinization and to examine the effect of the main component of green tea on the improvement of keratinization in rat oral mucosal preparations. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Histological and immunohistochemical analyses and quantitative mRNA measurements of caspase-14 and its substrate filaggrin were performed using different types of rat epithelial tissue and organotypic reconstruction culture models derived from epithelial cells and fibroblasts taken from the rat oral mucosa. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
In the skin, palate, buccal mucosa and esophagus, the degree of keratinization appeared to be associated with expression of cytokeratin 10. The relative protein and mRNA expression levels of caspase-14 and filaggrin were consistent with the degree of keratinization in the following order: skin > palate > buccal mucosa > esophagus. The culture models of palatal and buccal mucosa retained a stratified epithelial structure. Expression of caspase-14 appeared to be stronger in the palatal model than in the buccal model. Remarkably, epigallocatechin-3-gallate (EGCG) improved the localization of cytokeratins and increased the expression of caspase-14 and filaggrin. This expression was more intense in the palatal model than in the buccal model, indicating that both models maintain the intrinsic properties of keratinization of the mucosa from where the cultured cells were derived. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
These results suggest that keratinization is closely associated with expression of caspase-14 and filaggrin. Our reconstruction models are promising tools for drug evaluation and show that EGCG is beneficial for improving both keratinization and expression of the linked protease in the oral mucosa. |
| 学位名 |
|
|
学位名 |
博士（歯学） |
| 学位授与大学 |
|
|
|
学位授与機関識別子 |
37114 |
|
|
学位授与機関名 |
福岡歯科大学 |
| 学位授与年度 |
|
|
内容記述 |
2013年度 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2014-03-15 |
| 報告番号 |
|
|
学位授与番号 |
甲第246号 |
| 情報源 |
|
|
|
関連名称 |
J Periodontal Res. 2013 Dec 11. doi: 10.1111/jre.12152. |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
甲第246号_論文(村上) (21.4 MB)
