| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2024-11-18 |
| タイトル |
|
|
タイトル |
Creating 3D constructs with cranial neural crest-derived cell lines using a bio-3D printer |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
3D constructs |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
spheroid |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Bio-3D printer |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Tenascin C |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
田口, 雅英
吉本, 尚平
陶山, 可奈子
隅, 聡子
大木, 調
緒方, 佳代子
藤本, 亮太
村田, 大紀
中山, 功一
岡, 暁子
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Objectives: The development of bio-three-dimensional (bio-3D) printers has led to significant advances in regenerative medicine. Three-dimensional constructs, including spheroids, are maintained by extracellular matrix proteins secreted by cells so that the cells can be cultured in conditions closer to the physiological environment. This study aimed to create a useful 3D construct as a model of the dentin-pulp complex.
Methods: We examined the expression patterns of extracellular matrix proteins and cell proliferation areas in a 3D construct created using O9-1 cells derived from cranial neural crest cells of mice. The 3D construct was created by sticking the spheroid cultures onto a needle array using a bio-3D printer.
Results: Cell proliferation areas along with characteristic expression of tenascin C and DMP1 were evaluated. The expression of tenascin C and DMP1 was significantly enhanced in the spheroids compared to that in two-dimensional cultures. Moreover, cell proliferation regions and tenascin C expression were confirmed in the outer layer of spheroids in the embryonic stem cell medium, with insignificant DMP1 expression being observed. Interestingly, in a 3D construct cultured in calcification-induction medium, DMP1 expression was promoted, and DMP1-positive cells existed in the outermost layer without overlapping with tenascin C expression.
Conclusions: The extracellular matrix proteins, tenascin C and DMP1, were expressed in a polarized manner in spheroids and 3D constructs, similar to the findings in the dental papilla. Therefore, these 3D constructs show potential as artificial models for studying odontogenesis. |
|
言語 |
en |
| 学位名 |
|
|
言語 |
ja |
|
学位名 |
博士（歯学） |
| 学位授与大学 |
|
|
|
学位授与機関識別子Scheme |
kakenhi |
|
|
学位授与機関識別子 |
37114 |
|
|
言語 |
ja |
|
|
学位授与機関名 |
福岡歯科大学 |
| 学位授与年度 |
|
|
内容記述 |
2024年度 |
|
言語 |
ja |
| 学位授与年月日 |
|
|
学位授与年月日 |
2024-06-28 |
| 報告番号 |
|
|
学位授与番号 |
甲第352号 |
| 情報源 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.job.2024.05.005 |
|
|
言語 |
en |
|
|
関連名称 |
ScienceDirect |
| 関連サイト |
|
|
|
識別子タイプ |
ARK |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
甲第352号_論文審査結果の要旨(田口雅英).pdf (209 KB)
.png)
