| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2019-04-11 |
| タイトル |
|
|
タイトル |
Serum affects keratinization and tight junctions in three-dimensional cultures of the mouse keratinocyte cell line COCA through retinoic acid receptor-mediated signaling. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
retinoic acid receptor |
| キーワード |
|
|
主題 |
keratinocyte |
| キーワード |
|
|
主題 |
three-dimensional culture |
| キーワード |
|
|
主題 |
keratinization |
| キーワード |
|
|
主題 |
tight junction |
| キーワード |
|
|
主題 |
claudin |
| キーワード |
|
|
言語 |
en |
|
主題 |
retinoic acid receptor |
| キーワード |
|
|
言語 |
en |
|
主題 |
keratinocyte |
| キーワード |
|
|
言語 |
en |
|
主題 |
three-dimensional culture |
| キーワード |
|
|
言語 |
en |
|
主題 |
keratinization |
| キーワード |
|
|
言語 |
en |
|
主題 |
tight junction |
| キーワード |
|
|
言語 |
en |
|
主題 |
claudin |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Ozaki, Akane
Otani, Takahito
Kitagawa, Norio
Ogata, Kayoko
Iida, Hiroshi
Kojima, Hiroshi
Inai, Tetsuichiro
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Vitamin A, which is found in serum, is known to affect keratinocyte proliferation, epidermal differentiation, and keratinization. In mice, stratified epithelia in the oral cavity, esophagus, and forestomach are keratinized; however, these epithelia are not keratinized in humans. Several studies have reported that three-dimensional (3D) cultures of human keratinocytes in serum-containing medium could form keratinized epithelia. Here, we evaluated the effects of serum on the morphology, expression, and localization of differentiation markers and tight junction proteins, and paracellular permeability in 3D cultures of mouse keratinocytes. We found that only 0.1% calcium-depleted serum inhibited keratinization and induced a change in the expression of differentiation marker proteins from loricrin to keratin 4; the inhibition of retinoic acid receptor-mediated signaling reversed these changes. Furthermore, the serum reduced claudin-1 protein expression and prevented its localization at occludin-positive spots on the surface of 3D cultures. On the other hand, the serum increased the protein expression of claudin-4, occludin, zonula occludens-1, and E-cadherin. These changes may contribute to the reduction of the transepithelial electrical resistance by approximately half. In conclusion, mouse keratinocytes derived from the epidermis formed non-keratinized structures in 3D cultures in response to vitamin A in serum. The results suggest that retinoic acid receptor-mediated signaling may be inhibited in the mouse epithelia in the oral cavity, esophagus, and forestomach as well as the epidermis, leading to the keratinization of these epithelia. |
| 学位名 |
|
|
学位名 |
博士（歯学） |
| 学位授与大学 |
|
|
|
学位授与機関識別子 |
37114 |
|
|
学位授与機関名 |
福岡歯科大学 |
| 学位授与年度 |
|
|
内容記述 |
2018年度 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2019-03-02 |
| 報告番号 |
|
|
学位授与番号 |
甲第306号 |
| 関連サイト |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1007/s00418-018-1741-2 |
|
|
関連名称 |
SpringerLink |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
甲第306号_論文(尾崎茜) (1.9 MB)
