{"created":"2023-05-15T09:58:10.599062+00:00","id":99,"links":{},"metadata":{"_buckets":{"deposit":"b8ea92bb-214a-4f1d-86e6-4477ea234a0f"},"_deposit":{"created_by":15,"id":"99","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"99"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000099","sets":["1:20:21"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-11-30"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2018年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND:\nInteractions of resident bacteria and/or their producing lipopolysaccharide (LPS) with sulcular epithelial keratinocytes may be regulated by autophagy in the gingival sulcus. In this study, we investigated an induction of bacterial autophagy in exfoliative sulcular keratinocytes of the gingival sulcus and cultured keratinocytes treated with Porphyromonas gingivalis-originated LPS (PgLPS).","subitem_description_type":"Abstract"},{"subitem_description":"RESULTS:\nExfoliative sulcular keratinocytes showed an induction of autophagy, in addition to increased expression of LPS-mediated factors including lipopolysaccharide-binding protein and toll-like receptors (TLRs), leading to co-localization of bacteria with autophagosomes. In contrast, exfoliative keratinocytes from the free gingiva did not show similar autophagy. Autophagy activity in human cultured keratinocyte cells (HaCaT) was induced by PgLPS, which was dependent partially on the AMP-activated protein kinase (AMPK) pathway via increased intracellular reactive oxygen species (ROS) and was in association with an activation of TLR4 signaling. After incubation of cultured keratinocytes with E.coli BioParticles following PgLPS stimulation, co-localization of bioparticles with autophagosomes was enhanced. Conversely, blockage of autophagy with 3-methyladenin and LPS-binding with polymyxin B led to significant reduction of co-localization of particles with autophagosomes.","subitem_description_type":"Abstract"},{"subitem_description":"CONCLUSION:\nThese findings indicate that PgLPS-induced autophagy is at least partially responsible for interaction between bacteria and sulcular keratinocytes in the gingival sulcus.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第303号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"BMC"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://dx.doi.org/10.1186%2Fs12860-018-0168-x","subitem_relation_type_select":"URI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kanako, Hagio-Izaki"}]},{"creatorNames":[{"creatorName":"Madoka, Yasunaga"}]},{"creatorNames":[{"creatorName":"Masahiro, Yamaguchi"}]},{"creatorNames":[{"creatorName":"Hiroshi, Kajiya"}]},{"creatorNames":[{"creatorName":"Hiromitsu, Morita"}]},{"creatorNames":[{"creatorName":"Masahiro, Yoneda"}]},{"creatorNames":[{"creatorName":"Takao, Hirofuji"}]},{"creatorNames":[{"creatorName":"Jun, Ohno"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-02-13"}],"displaytype":"detail","filename":"甲第303号_論文(伊﨑佳那子).pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"甲第303号_論文(伊﨑佳那子)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/99/files/甲第303号_論文(伊﨑佳那子).pdf"},"version_id":"8bc29fb7-db04-4e45-9ef6-f77b042898ae"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-02-13"}],"displaytype":"detail","filename":"甲第303号_論文内容の要旨(伊﨑佳那子).pdf","filesize":[{"value":"142.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"甲第303号_論文内容の要旨(伊﨑佳那子)","url":"https://fdc.repo.nii.ac.jp/record/99/files/甲第303号_論文内容の要旨(伊﨑佳那子).pdf"},"version_id":"64ed7164-da67-48e4-be8d-28ccfb275f71"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-02-13"}],"displaytype":"detail","filename":"甲第303号_論文審査結果の要旨(伊﨑佳那子).pdf","filesize":[{"value":"179.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"甲第303号_論文審査結果の要旨(伊﨑佳那子)","url":"https://fdc.repo.nii.ac.jp/record/99/files/甲第303号_論文審査結果の要旨(伊﨑佳那子).pdf"},"version_id":"38d14230-b9d8-4ba5-9ab3-2d38fc152316"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"歯肉溝"},{"subitem_subject":"オートファジー"},{"subitem_subject":"ポルフィロモナスジンジバリス"},{"subitem_subject":"リポポリサッカライド"},{"subitem_subject":"活性酸素"},{"subitem_subject":"Gingival sulcus","subitem_subject_language":"en"},{"subitem_subject":"Autophagy","subitem_subject_language":"en"},{"subitem_subject":"Porphyromonas gingivalis","subitem_subject_language":"en"},{"subitem_subject":"Lipopolysaccharide","subitem_subject_language":"en"},{"subitem_subject":"Reactive oxygen species","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["21"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-02-13"},"publish_date":"2019-02-13","publish_status":"0","recid":"99","relation_version_is_last":true,"title":["Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-06-25T22:32:50.037641+00:00"}