{"created":"2023-05-15T09:58:09.675164+00:00","id":70,"links":{},"metadata":{"_buckets":{"deposit":"62a95acc-fcee-4dfb-8f1e-8d22f1ea66ed"},"_deposit":{"created_by":15,"id":"70","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"70"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000070","sets":["1:14:15"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2017-03-11"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2016年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In tooth root development, periodontal ligament (PDL) and cementum are formed by the coordination with the fragmentation of Hertwig's epithelial root sheath (HERS) and the differentiation of dental follicle mesenchymal cells. However, the function of the dental epithelial cells after HERS fragmentation in the PDL is not fully understood. Here, we found that TGF-β regulated HERS fragmentation via epithelial-mesenchymal transition (EMT), and the fragmented epithelial cells differentiated into PDL fibroblastic cells with expressing of PDL extracellular matrix (ECM). In the histochemical analysis, TGF-β was expressed in odontoblast layer adjacent of HERS during root development. Periostin expression was detected around fragmented epithelial cells on the root surface, but not in HERS. In the experiment using an established mouse HERS cell line (HERS01a), TGF-β1 treatment decreased E-cadherin and relatively increased N-cadherin expression. TGF-β1 treatment in HERS01a induced further expression of important ECM proteins for acellular cementum and PDL development such as fibronectin and periostin. Taken together, activation of TGF-βsignaling induces HERS fragmentation through EMT and the fragmented HERS cells contribute to formation of PDL and acellular cementum through periostin and fibronectin expression.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第284号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"J-STAGE"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://doi.org/10.2220/biomedres.38.61","subitem_relation_type_select":"DOI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Itaya, Satoshi"}]},{"creatorNames":[{"creatorName":"Oka, Kyoko"}]},{"creatorNames":[{"creatorName":"Ogata, Kayoko"}]},{"creatorNames":[{"creatorName":"Tamura, Shougo"}]},{"creatorNames":[{"creatorName":"Kira-Tatsuoka, Michiko"}]},{"creatorNames":[{"creatorName":"Fujiwara, Naoki"}]},{"creatorNames":[{"creatorName":"Otsu, Keishi"}]},{"creatorNames":[{"creatorName":"Tsuruga, Eichi"}]},{"creatorNames":[{"creatorName":"Ozaki, Masao"}]},{"creatorNames":[{"creatorName":"Harada, Hidemitsu"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-29"}],"displaytype":"detail","filename":"甲第284号_論文(板家).pdf","filesize":[{"value":"3.6 MB"}],"format":"application/pdf","licensetype":"甲第284号_論文(板家).pdf","mimetype":"application/pdf","url":{"label":"甲第284号_論文(板家)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/70/files/甲第284号_論文(板家).pdf"},"version_id":"99122c84-898f-4574-8990-83e3433052df"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-29"}],"displaytype":"detail","filename":"甲第284号_論文内容の要旨(板家).pdf","filesize":[{"value":"245.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://fdc.repo.nii.ac.jp/record/70/files/甲第284号_論文内容の要旨(板家).pdf"},"version_id":"bfc62e5e-9b6c-4a98-950f-f37d934615f6"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-29"}],"displaytype":"detail","filename":"甲第284号_論文審査結果の要旨(板家).pdf","filesize":[{"value":"207.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査結果の要旨","url":"https://fdc.repo.nii.ac.jp/record/70/files/甲第284号_論文審査結果の要旨(板家).pdf"},"version_id":"bea722ec-eb19-4702-87da-261f2e0e9942"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ヘルトヴィッヒ上皮鞘"},{"subitem_subject":"上皮間葉転換"},{"subitem_subject":"TGF-β"},{"subitem_subject":"Hertwig's epithelial root sheath","subitem_subject_language":"en"},{"subitem_subject":"TGF-β","subitem_subject_language":"en"},{"subitem_subject":"Alkaline Phosphatase","subitem_subject_language":"en"},{"subitem_subject":"Animals","subitem_subject_language":"en"},{"subitem_subject":"Cadherins","subitem_subject_language":"en"},{"subitem_subject":"Cell Adhesion","subitem_subject_language":"en"},{"subitem_subject":"Cell Adhesion Molecules","subitem_subject_language":"en"},{"subitem_subject":"Cell Differentiation","subitem_subject_language":"en"},{"subitem_subject":"Cell Line","subitem_subject_language":"en"},{"subitem_subject":"Collagen Type I","subitem_subject_language":"en"},{"subitem_subject":"Core Binding Factor Alpha 1 Subunit","subitem_subject_language":"en"},{"subitem_subject":"Dental Cementum","subitem_subject_language":"en"},{"subitem_subject":"Epithelial Cells","subitem_subject_language":"en"},{"subitem_subject":"Epithelial-Mesenchymal Transition","subitem_subject_language":"en"},{"subitem_subject":"Extracellular Matrix","subitem_subject_language":"en"},{"subitem_subject":"Fibronectins","subitem_subject_language":"en"},{"subitem_subject":"Gene Expression Regulation","subitem_subject_language":"en"},{"subitem_subject":"Mice","subitem_subject_language":"en"},{"subitem_subject":"Odontoblasts","subitem_subject_language":"en"},{"subitem_subject":"Osteocalcin","subitem_subject_language":"en"},{"subitem_subject":"Periodontal Ligament","subitem_subject_language":"en"},{"subitem_subject":"Tooth Root","subitem_subject_language":"en"},{"subitem_subject":"Transcription Factors","subitem_subject_language":"en"},{"subitem_subject":"Transforming Growth Factor beta1","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Hertwig's epithelial root sheath cells contribute to formation of periodontal ligament through epithelial-mesenchymal transition by TGF-β.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Hertwig's epithelial root sheath cells contribute to formation of periodontal ligament through epithelial-mesenchymal transition by TGF-β.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["15"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-03-29"},"publish_date":"2017-03-29","publish_status":"0","recid":"70","relation_version_is_last":true,"title":["Hertwig's epithelial root sheath cells contribute to formation of periodontal ligament through epithelial-mesenchymal transition by TGF-β."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-06-16T00:39:53.641726+00:00"}