{"created":"2023-05-15T09:58:07.257581+00:00","id":7,"links":{},"metadata":{"_buckets":{"deposit":"e129f1e9-9d59-45e7-88d4-a6118f510985"},"_deposit":{"created_by":15,"id":"7","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"7"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000007","sets":["1:3:6"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2014-03-15"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2013年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Transforming growth factor-β1 (TGF-β1) reportedly causes the differentiation of fibroblasts to myofibroblasts during wound healing. We investigated the mechanism underlying the activation of latent TGF-β1 released by keratinocytes in efforts to identify promising pharmacological approaches for the prevention of hypertrophic scar formation. A three-dimensional collagen gel matrix culture was prepared using rat keratinocytes and dermal fibroblasts. Stratified keratinocytes promoted the TGF receptor-dependent increase in α-smooth muscle actin (α-SMA) immunostaining and mRNA levels in fibroblasts. Latent TGF-β1 was found to be localized suprabasally and secreted. α-SMA expression was inhibited by an anti-αv-integrin antibody and a matrix metalloproteinase (MMP) inhibitor, GM6001. In a two-dimensional fibroblast culture, α-SMA expression depended on the production of endogenous TGF-β1 and required αv-integrin or MMP for the response to recombinant latent TGF-β1. In keratinocyte-conditioned medium, MMP-dependent latent TGF-β1 secretion was detected. Applying this medium to the fibroblast culture enhanced α-SMA production. This effect was decreased by GM6001, the anti-αv-integrin antibody, or the preabsorption of latent TGF-β1. These results indicate that keratinocytes secrete latent TGF-β1, which is liberated to fibroblasts over distance and is activated to produce α-SMA with the aid of a positive-feedback loop. MMP inhibition was effective for targeting both keratinocytes and fibroblasts in this model.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第253号"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"J Pharmacol Sci. 2014;124(2):230-43. Epub 2014 Jan 31."}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"J-STAGE"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/article/jphs/124/2/124_13209FP/_article","subitem_relation_type_select":"URI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hata, Shozaburo"}]},{"creatorNames":[{"creatorName":"Okamura, Kazuhiko"}]},{"creatorNames":[{"creatorName":"Hatta, Mitsutoki"}]},{"creatorNames":[{"creatorName":"Ishikawa, Hiroyuki"}]},{"creatorNames":[{"creatorName":"Yamazaki, Jun"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-08-20"}],"displaytype":"detail","filename":"甲第253号_論文(秦).pdf","filesize":[{"value":"5.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第253号_論文(秦)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/7/files/甲第253号_論文(秦).pdf"},"version_id":"045f8571-a878-452d-ab76-b4648faafcd4"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-04-28"}],"displaytype":"detail","filename":"甲第253号_論文内容の要旨(秦).pdf","filesize":[{"value":"89.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://fdc.repo.nii.ac.jp/record/7/files/甲第253号_論文内容の要旨(秦).pdf"},"version_id":"266d86f8-e0fa-4394-b538-ef097bf5dd16"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-04-28"}],"displaytype":"detail","filename":"甲第253号_論文審査結果の要旨(秦).pdf","filesize":[{"value":"297.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査結果の要旨","url":"https://fdc.repo.nii.ac.jp/record/7/files/甲第253号_論文審査結果の要旨(秦).pdf"},"version_id":"8462f091-5fa1-4165-ad92-64b592695bfe"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"keratinocyte","subitem_subject_language":"en"},{"subitem_subject":"fibroblast","subitem_subject_language":"en"},{"subitem_subject":"transforming growth factor-β1","subitem_subject_language":"en"},{"subitem_subject":"αv-integrin","subitem_subject_language":"en"},{"subitem_subject":"matrix metalloproteinase","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Proteolytic and non-proteolytic activation of keratinocyte-derived latent TGF-β1 induces fibroblast differentiation in a wound-healing model using rat skin.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Proteolytic and non-proteolytic activation of keratinocyte-derived latent TGF-β1 induces fibroblast differentiation in a wound-healing model using rat skin.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["6"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-04-28"},"publish_date":"2014-04-28","publish_status":"0","recid":"7","relation_version_is_last":true,"title":["Proteolytic and non-proteolytic activation of keratinocyte-derived latent TGF-β1 induces fibroblast differentiation in a wound-healing model using rat skin."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-06-24T01:54:51.772007+00:00"}