{"created":"2023-05-15T09:58:08.801923+00:00","id":47,"links":{},"metadata":{"_buckets":{"deposit":"81644f3c-d307-4183-8037-671ead271bd7"},"_deposit":{"created_by":15,"id":"47","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"47"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000047","sets":["1:7:8"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-03-14"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2014年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND:\nCLCA was postulated to be a calcium-activated chloride channel accessory protein. Recent reports indicate that CLCA isoforms are likely to be expressed in different layers of the stratified epithelium of the skin.\n\nOBJECTIVE:\nThe present study investigated the transcriptional mechanism by which murine CLCA2 (mCLCA2) is expressed in the transformed keratinocyte line Pam212 that can differentiate.\n\nMETHODS:\nA luciferase reporter assay, chromatin immunoprecipitation (ChIP) assay, reverse transcription-PCR, and immunocytochemistry were performed using Pam212 cells.\n\nRESULTS:\nPromoter activity of mCLCA2 was inhibited profoundly by site-directed mutagenesis of a putative nuclear factor-κB (NF-κB) binding site and by treatment with siRNA against p65. ChIP and transcription factor assays showed the specific association of endogenously activated p65 protein with the NF-κB binding domain. As confirmed by the nuclear translocation of p65, tumor necrosis factor α and caffeic acid phenethyl ester (CAPE) increased and decreased mCLCA2 promoter activity, respectively, but exhibited modest effects on endogenous mCLCA2 expression in cells in culture medium containing 0.05 mM Ca(2+). When the Ca(2+) concentration was raised to 1.0mM, the mRNA and protein levels of mCLCA2 increased as well as those of the differentiation markers keratin 1 (K1) and K10. CAPE profoundly suppressed only the Ca(2+)-triggered expression of mCLCA2, not K1 or K10. Immunohistochemistry of native skin and organotypic 3D cultures confirmed the distribution of the CLCA2 homolog in differentiated cells.\n\nCONCLUSION:\nThe present study revealed for the first time that basal NF-κB activity is involved in the Ca(2+)-dependent regulation of mCLCA2 expression in a mouse keratinocyte line.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第270号"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"J Dermatol Sci. 2015 Jun;78(3):189-96. doi: 10.1016/j.jdermsci.2015.03.007. Epub 2015 Mar 18."}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"ScienceDirect"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.sciencedirect.com/science/article/pii/S0923181115000857","subitem_relation_type_select":"URI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hiromatsu, Ryo"}]},{"creatorNames":[{"creatorName":"Hatta, Mitsutoki"}]},{"creatorNames":[{"creatorName":"Okamura, Kazuhiko"}]},{"creatorNames":[{"creatorName":"Sakagami, Ryuji"}]},{"creatorNames":[{"creatorName":"Yamazaki, Jun"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-07-22"}],"displaytype":"detail","filename":"甲第270号_論文(廣松).pdf","filesize":[{"value":"2.8 MB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第270号_論文(廣松)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/47/files/甲第270号_論文(廣松).pdf"},"version_id":"1dfc5959-2ec5-4740-a310-3413f6d2dfbc"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-07-22"}],"displaytype":"detail","filename":"甲第270号_論文内容の要旨(廣松).pdf","filesize":[{"value":"313.7 kB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://fdc.repo.nii.ac.jp/record/47/files/甲第270号_論文内容の要旨(廣松).pdf"},"version_id":"3e2b96eb-a7c7-4d58-908a-2d917cdd171d"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-07-22"}],"displaytype":"detail","filename":"甲第270号_論文審査結果の要旨(廣松).pdf","filesize":[{"value":"291.5 kB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査結果の要旨","url":"https://fdc.repo.nii.ac.jp/record/47/files/甲第270号_論文審査結果の要旨(廣松).pdf"},"version_id":"0a8c7dbd-6423-4741-aa91-589a2aac5046"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"転写制御"},{"subitem_subject":"ケラチノサイト"},{"subitem_subject":"CLCA","subitem_subject_language":"en"},{"subitem_subject":"NF-kB","subitem_subject_language":"en"},{"subitem_subject":"transcriptioal control","subitem_subject_language":"en"},{"subitem_subject":"keratinocyte","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"NF-κB-regulated transcriptional control of CLCA in a differentiated mouse keratinocyte line.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"NF-κB-regulated transcriptional control of CLCA in a differentiated mouse keratinocyte line.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["8"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-07-22"},"publish_date":"2015-07-22","publish_status":"0","recid":"47","relation_version_is_last":true,"title":["NF-κB-regulated transcriptional control of CLCA in a differentiated mouse keratinocyte line."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-11T01:22:17.032466+00:00"}