{"created":"2023-05-15T09:58:08.264045+00:00","id":36,"links":{},"metadata":{"_buckets":{"deposit":"23e61d62-250f-4804-8915-d877d9a04944"},"_deposit":{"created_by":15,"id":"36","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"36"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000036","sets":["1:7:8"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-03-14"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2014年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Epigenetic modifications play important roles in the regulation of gene expression determining cellular phenotype as well as various pathologies such as cancer. Although the loss of keratin 13 (KRT13) is reportedly linked to malignant transformation of oral epithelial cells, the molecular mechanisms through which KRT13 is repressed in oral squamous cell carcinoma (OSCC) remain unclear. The aim of this study is to identify the epigenetic alterations of the KRT13 gene in OSCCs.","subitem_description_type":"Abstract"},{"subitem_description":"We investigated KRT13 expression levels and chromatin modifications of the KRT13 promoter in the three OSCC cell lines (HSC4, HSC3, and SAS). The expression levels of KRT13 protein and mRNA were analyzed by western blotting and quantitative reverse-transcription polymerase chain reaction, respectively, and the localization of KRT13 protein was detected by immunofluorescence. DNA methylation and histone modifications in the KRT13 promoter were determined by bisulfite sequencing and chromatin immunoprecipitation (ChIP), respectively. For the pharmacological depletion of Polycomb repressive complex 2 (PRC2), cells were treated with 3-deazaneplanocin A (DZNep).","subitem_description_type":"Abstract"},{"subitem_description":"KRT13 expression was transcriptionally silenced in the HSC3 and SAS cells and post-transcriptionally repressed in the HSC4 cells, while the KRT13 promoter was hypermethylated in all of the three OSCC cell lines. ChIP analysis revealed that PRC2-mediated trimethylation of Lys 27 on histone H3 (H3K27me3) was increased in the KRT13 promoter in the HSC3 and SAS cells. Finally, we demonstrated that the treatment of SAS cells with DZNep reactivated the transcription of KRT13 gene.","subitem_description_type":"Abstract"},{"subitem_description":"Our data provide mechanistic insights into the epigenetic silencing of KRT13 genes in OSCC cells and might be useful for the development of diagnostic markers and novel therapeutic approaches against OSCCs.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第266号"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"BMC Cancer. 2014 Dec 20;14:988. doi: 10.1186/1471-2407-14-988."}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"BMC"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.biomedcentral.com/1471-2407/14/988","subitem_relation_type_select":"URI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Naganuma, Kaori"}]},{"creatorNames":[{"creatorName":"Hatta, Mitsutoki"}]},{"creatorNames":[{"creatorName":"Ikebe, Tetsuro"}]},{"creatorNames":[{"creatorName":"Yamazaki, Jun"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-03-16"}],"displaytype":"detail","filename":"甲第266号_論文(永沼).pdf","filesize":[{"value":"1.6 MB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第266号_論文(永沼)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/36/files/甲第266号_論文(永沼).pdf"},"version_id":"36d26d40-6f90-4789-b391-f5076bf9f852"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-03-16"}],"displaytype":"detail","filename":"甲第266号_論文内容の要旨(永沼).pdf","filesize":[{"value":"314.9 kB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://fdc.repo.nii.ac.jp/record/36/files/甲第266号_論文内容の要旨(永沼).pdf"},"version_id":"e0a9f096-19e2-4305-9e76-5265020b9621"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-03-16"}],"displaytype":"detail","filename":"甲第266号_論文審査結果の要旨(永沼).pdf","filesize":[{"value":"244.4 kB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査結果の要旨","url":"https://fdc.repo.nii.ac.jp/record/36/files/甲第266号_論文審査結果の要旨(永沼).pdf"},"version_id":"2abe8d0f-9d56-46da-a3cf-245c927f421f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Keratin 13 (KRT13)","subitem_subject_language":"en"},{"subitem_subject":"Oral squamous cell carcinoma (OSCC)","subitem_subject_language":"en"},{"subitem_subject":"Polycomb repressive complex 2 (PRC2)","subitem_subject_language":"en"},{"subitem_subject":"Gene silencing","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Epigenetic alterations of the keratin 13 gene in oral squamous cell carcinoma.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Epigenetic alterations of the keratin 13 gene in oral squamous cell carcinoma.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["8"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-03-16"},"publish_date":"2015-03-16","publish_status":"0","recid":"36","relation_version_is_last":true,"title":["Epigenetic alterations of the keratin 13 gene in oral squamous cell carcinoma."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-11T01:22:03.202113+00:00"}