{"created":"2023-05-15T09:58:08.219644+00:00","id":35,"links":{},"metadata":{"_buckets":{"deposit":"cb22a080-a1d8-46d7-bc83-2b96e9557a58"},"_deposit":{"created_by":15,"id":"35","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"35"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000035","sets":["1:7:8"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-03-14"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2014年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BackgroundMesenchymal stem cells (MSCs) are a favored cell source for regenerative medicine because of their multilinage potential. However, the conventional monolayer technique used to culture MSCs, inadequately overcomes their low differentiation capacity. Culture of MSCs in multicellular spheroids, more accurately mimics the in-vivo microenvironment; thus, resolving this problem. In this study, we assessed whether the osteoregeneartive potential of MSC spheroids is greater than that of monolayer MSCs.ResultsMSC spheroids were generated from rat MSCs (rMSCs) using low-binding plates. Real-time reverse transcription-polymerase chain reaction and immunocytochemical analysis indicated that osteogenic properties were accelerated in MSC spheroids compared with monolayer rMSCs when treated with an osteoblast-inducer reagent for 7 days. Moreover, increased calcium deposition was visualized in MSC spheroids using Alizarin red staining. In a rat calvarial defect model, micro-computed tomography and histological assays showed that MSC spheroid-engrafted defects experienced enhanced bone regeneration.ConclusionsOur in-vitro and in-vivo results reveal that MSCs in the spheroid culture exhibit enhanced osteoregenerative efficiency compared with monolayer MSCs.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第263号"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"BMC Biotechnol. 2014 Dec 6;14(1):105. "}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"BMC"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.biomedcentral.com/1472-6750/14/105","subitem_relation_type_select":"URI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamaguchi, Yuichiro"}]},{"creatorNames":[{"creatorName":"Ohno, Jun"}]},{"creatorNames":[{"creatorName":"Sato, Ayako"}]},{"creatorNames":[{"creatorName":"Kido, Hirofumi"}]},{"creatorNames":[{"creatorName":"Fukushima, Tadao"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-03-16"}],"displaytype":"detail","filename":"甲第263号_論文(山口).pdf","filesize":[{"value":"3.2 MB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第263号_論文(山口)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/35/files/甲第263号_論文(山口).pdf"},"version_id":"06510859-10c6-4783-826f-8667c522be29"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-03-16"}],"displaytype":"detail","filename":"甲第263号_論文内容の要旨(山口).pdf","filesize":[{"value":"316.5 kB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://fdc.repo.nii.ac.jp/record/35/files/甲第263号_論文内容の要旨(山口).pdf"},"version_id":"156d04b9-ed2d-444b-b807-7ed9fb51baa5"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-03-16"}],"displaytype":"detail","filename":"甲第263号_論文審査結果の要旨(山口).pdf","filesize":[{"value":"257.8 kB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査結果の要旨","url":"https://fdc.repo.nii.ac.jp/record/35/files/甲第263号_論文審査結果の要旨(山口).pdf"},"version_id":"5d7c983d-35b2-479f-b88c-98ec21b136b9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"スフェロイド"},{"subitem_subject":"間葉系幹細胞"},{"subitem_subject":"骨形成"},{"subitem_subject":"ラット頭蓋骨骨欠損"},{"subitem_subject":"骨再生"},{"subitem_subject":"Spheroid","subitem_subject_language":"en"},{"subitem_subject":"Mesenchymal stem cell","subitem_subject_language":"en"},{"subitem_subject":"Osteogenesis","subitem_subject_language":"en"},{"subitem_subject":"Rat calvarial defect","subitem_subject_language":"en"},{"subitem_subject":"Bone regeneration","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Mesenchymal stem cell spheroids exhibit enhanced in-vitro and in-vivo osteoregenerative potential.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Mesenchymal stem cell spheroids exhibit enhanced in-vitro and in-vivo osteoregenerative potential.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["8"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-03-16"},"publish_date":"2015-03-16","publish_status":"0","recid":"35","relation_version_is_last":true,"title":["Mesenchymal stem cell spheroids exhibit enhanced in-vitro and in-vivo osteoregenerative potential."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-11T01:21:56.991912+00:00"}