{"created":"2023-05-15T09:58:08.136059+00:00","id":33,"links":{},"metadata":{"_buckets":{"deposit":"f385a3b5-d252-4619-a77b-71c80e828604"},"_deposit":{"created_by":15,"id":"33","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"33"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000033","sets":["1:7:8"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-03-14"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2014年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To investigate the involvement of stress-activated protein kinases, JNK and p38 MAPK, in the assembly of tight junctions in keratinocytes, we treated HaCaT cells with various combinations of SP600125 (an inhibitor of JNK), SB202190 (an inhibitor of p38 MAPK) and anisomycin (an activator of both JNK and p38 MAPK) and examined the localization of ZO-1, an undercoat constitutive protein of the tight junction. Short-term (8h) incubation with SP600125, SB202190 or anisomycin induced the accumulation of ZO-1 in the cell-cell contacts, with reduced ZO-1 staining in the cytoplasm, while only long-term (24h) incubation with SP600125 induced the accumulation of ZO-1. SP600125, SB202190 or SP600125 plus SB202190 treatment induced thin linear staining for ZO-1 in the cell-cell contacts. Anisomycin treatment induced thick and irregular linear staining for ZO-1, while anisomycin plus SP600125 treatment induced zipper-like staining for ZO-1. Anisomycin plus SB202190 treatment or anisomycin plus both SP600125 and SB202190 treatment for 8h failed to lead to the accumulation of ZO-1 in cell-cell contacts, but induced thin linear staining with several gaps 16h after removal of these agents. These results suggest that the localization of ZO-1 in cell-cell contacts is differently regulated by activation and inhibition of JNK and/or p38 MAPK depending on the incubation period.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第268号"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Tissue Cell. 2015 Feb;47(1):1-9. doi: 10.1016/j.tice.2014.10.001. Epub 2014 Oct 14."}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"        doi:10.1016/j.tice.2014.10.001"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.tice.2014.10.001","subitem_relation_type_select":"DOI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Minakami, Masahiko"}]},{"creatorNames":[{"creatorName":"Kitagawa, Norio"}]},{"creatorNames":[{"creatorName":"Iida, Hiroshi"}]},{"creatorNames":[{"creatorName":"Anan, Hisashi"}]},{"creatorNames":[{"creatorName":"Inai, Tetsuichiro"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-08-04"}],"displaytype":"detail","filename":"甲第268号_論文(水上).pdf","filesize":[{"value":"1.2 MB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第268号_論文(水上)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/33/files/甲第268号_論文(水上).pdf"},"version_id":"4ad69f5e-240d-4a1c-83be-a7c24531ff6a"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-03-16"}],"displaytype":"detail","filename":"甲第268号_論文内容の要旨(水上).pdf","filesize":[{"value":"331.0 kB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://fdc.repo.nii.ac.jp/record/33/files/甲第268号_論文内容の要旨(水上).pdf"},"version_id":"d6fb7e41-1688-481b-a124-ff1e5a864fc2"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-03-16"}],"displaytype":"detail","filename":"甲第268号_論文審査結果の要旨(水上).pdf","filesize":[{"value":"258.8 kB"}],"format":"binary/octet-stream","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査結果の要旨","url":"https://fdc.repo.nii.ac.jp/record/33/files/甲第268号_論文審査結果の要旨(水上).pdf"},"version_id":"1f46e1d4-4244-4d97-981b-acfc5cf47b15"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"MAPK"},{"subitem_subject":"JNK"},{"subitem_subject":"p38"},{"subitem_subject":"タイト結合"},{"subitem_subject":"ZO-1"},{"subitem_subject":"HaCaT細胞"},{"subitem_subject":"mitogen-activated protein kinase (MAPK)","subitem_subject_language":"en"},{"subitem_subject":"c-Jun NH2-terminal protein kinase (JNK)","subitem_subject_language":"en"},{"subitem_subject":"p38 MAPK","subitem_subject_language":"en"},{"subitem_subject":"tight junction","subitem_subject_language":"en"},{"subitem_subject":"ZO-1","subitem_subject_language":"en"},{"subitem_subject":"HaCaT cell","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"p38 Mitogen-activated protein kinase and c-Jun NH2-terminal protein kinase regulate the accumulation of a tight junction protein, ZO-1, in cell-cell contacts in HaCaT cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"p38 Mitogen-activated protein kinase and c-Jun NH2-terminal protein kinase regulate the accumulation of a tight junction protein, ZO-1, in cell-cell contacts in HaCaT cells.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["8"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-03-16"},"publish_date":"2015-03-16","publish_status":"0","recid":"33","relation_version_is_last":true,"title":["p38 Mitogen-activated protein kinase and c-Jun NH2-terminal protein kinase regulate the accumulation of a tight junction protein, ZO-1, in cell-cell contacts in HaCaT cells."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-07-11T01:21:50.929822+00:00"}