{"created":"2023-05-15T09:58:07.345609+00:00","id":11,"links":{},"metadata":{"_buckets":{"deposit":"9ac01914-ad90-4d6d-9ada-e8996ad4d744"},"_deposit":{"created_by":15,"id":"11","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"11"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000011","sets":["1:3:6"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2014-03-15"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2013年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The ciliary zonule, also known as Zinn's zonule, is composed of oxytalan fibers. However, the mechanism by which epithelial cells in the ciliary body form these fibers in not fully understood. We examined human nonpigmented ciliary epithelial cells to determine the appearance and amount of oxytalan fibers in terms of positivity for their major components, fibrillin-1 and fibrillin-2. Examination of fibrillin-1 and fibrillin-2 expression by immunofluorescence revealed that thin fibers positive for fibrillin-1 on Day 2 changed to thick fibers by Day 8. The fibers positive for fibrillin-2 appeared on the thick fibrillin-1-positive fibers after Day 4. Northern blot analysis revealed that the level of fibrillin-1 did not change markedly, while induction of fibrillin-2 gene was evident on Day 5. Western blot analysis showed that fibrillin-1 deposition increased gradually, while that of fibrillin-2 increased markedly from Day 5 to Day 8. Fibrillin-1 suppression did not lead to the formation of fibrillin-2-positive thick fibers, whereas fibrillin-2 suppression led to the formation of fibrillin-1-positive thin fibers, but not thick fibers. These results suggest that both fibrillin-1 and fibrillin-2 are essential for the formation of thick oxytalan fibers in the ciliary zonule and are informative for clarifying the mechanism of homeostasis of the ocular matrix.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第255号"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Connect Tissue Res. 2012;53(1):14-20. doi: 10.3109/03008207.2011.602767. Epub 2011 Aug 18."}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"informa"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://informahealthcare.com/doi/abs/10.3109/03008207.2011.602767","subitem_relation_type_select":"URI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamanouchi, Kaori"}]},{"creatorNames":[{"creatorName":"Tsuruga, Eichi"}]},{"creatorNames":[{"creatorName":"Oka, Kyoko"}]},{"creatorNames":[{"creatorName":"Sawa, Yoshihiko"}]},{"creatorNames":[{"creatorName":"Ishikawa, Hiroyuki"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-04-28"}],"displaytype":"detail","filename":"甲第255号_論文(山之内).pdf","filesize":[{"value":"732.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第255号_論文(山之内)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/11/files/甲第255号_論文(山之内).pdf"},"version_id":"20e8a991-eec9-4de7-beb7-6f2e18b7ca27"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-04-28"}],"displaytype":"detail","filename":"甲第255号_論文内容の要旨(山之内).pdf","filesize":[{"value":"85.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://fdc.repo.nii.ac.jp/record/11/files/甲第255号_論文内容の要旨(山之内).pdf"},"version_id":"b8f0a263-db60-4cdc-9c6c-d82a762d27b5"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-04-28"}],"displaytype":"detail","filename":"甲第255号_論文審査結果の要旨(山之内).pdf","filesize":[{"value":"295.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"論文結果の要旨","url":"https://fdc.repo.nii.ac.jp/record/11/files/甲第255号_論文審査結果の要旨(山之内).pdf"},"version_id":"9ab66742-1fb9-42d6-8396-c7b8584a19ec"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Fibrillin-1","subitem_subject_language":"en"},{"subitem_subject":"Fibrillin-2","subitem_subject_language":"en"},{"subitem_subject":"microfibrils","subitem_subject_language":"en"},{"subitem_subject":"oxytalan fibers","subitem_subject_language":"en"},{"subitem_subject":"ciliary zonule","subitem_subject_language":"en"},{"subitem_subject":"Cells, Cultured","subitem_subject_language":"en"},{"subitem_subject":"Ciliary Body","subitem_subject_language":"en"},{"subitem_subject":"Epithelial Cells","subitem_subject_language":"en"},{"subitem_subject":"Extracellular Matrix","subitem_subject_language":"en"},{"subitem_subject":"Extracellular Matrix Proteins","subitem_subject_language":"en"},{"subitem_subject":"Gene Expression Regulation","subitem_subject_language":"en"},{"subitem_subject":"Gene Knockdown Techniques","subitem_subject_language":"en"},{"subitem_subject":"Humans","subitem_subject_language":"en"},{"subitem_subject":"Microfilament Proteins","subitem_subject_language":"en"},{"subitem_subject":"Pigmentation","subitem_subject_language":"en"},{"subitem_subject":"RNA, Small Interfering","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Fibrillin-1 and fibrillin-2 are essential for formation of thick oxytalan fibers in human nonpigmented ciliary epithelial cells in vitro.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Fibrillin-1 and fibrillin-2 are essential for formation of thick oxytalan fibers in human nonpigmented ciliary epithelial cells in vitro.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["6"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-04-28"},"publish_date":"2014-04-28","publish_status":"0","recid":"11","relation_version_is_last":true,"title":["Fibrillin-1 and fibrillin-2 are essential for formation of thick oxytalan fibers in human nonpigmented ciliary epithelial cells in vitro."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-06-24T01:54:57.462628+00:00"}