{"created":"2023-05-15T09:58:10.646386+00:00","id":100,"links":{},"metadata":{"_buckets":{"deposit":"b7d1727c-c050-418d-b297-899caa8f14f4"},"_deposit":{"created_by":15,"id":"100","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"100"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000100","sets":["1:20:21"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-04-27"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2018年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND:\nKeratinocytes release several factors that are involved in wound contracture and scar formation. We previously reported that a three-dimensional reconstruction model derived from rat skin represents a good wound healing model.\nOBJECTIVE:\nWe characterized the role of transient receptor potential (TRP) channels in the release of transforming growth factor (TGF)-β1 from keratinocytes and the differentiation of fibroblasts to identify possible promising pharmacological approaches to prevent scar formation and contractures.\n\nMETHODS:\nThe three-dimensional culture model was made from rat keratinocytes seeded on a collagen gel in which dermal fibroblasts had been embedded.\n\nRESULTS:\nAmong the TRP channel inhibitors tested, the TRPV2 inhibitors SKF96365 and tranilast attenuated most potently keratinocyte-dependent and - independent collagen gel contraction due to TGF-β signaling as well as TGF-β1 release from keratinocytes and α-smooth muscle actin production in myofibroblasts. Besides the low amounts detected in normal dermis, TRPV2 mRNA and protein levels were increased after fibroblasts were embedded in the gel. TRPV2 was also expressed in the epidermis and keratinocyte layers of the model. Both inhibitors and TRPV2 siRNA attenuated the intracellular increase of Ca2+ induced by the TRPV agonist 2-aminoethoxydiphenyl borate in TGF-β1-pretreated fibroblasts.\n\nCONCLUSION:\nThis is the first study to show that compounds targeting TRPV2 channels ameliorate wound contraction through the inhibition of TGF-β1 release and the differentiation of dermal fibroblasts in a culture model","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第299号"}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"ScienceDirect"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.jdermsci.2018.03.003","subitem_relation_type_select":"DOI"}}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ishii, Taro"}]},{"creatorNames":[{"creatorName":"Uchida, Kunitoshi"}]},{"creatorNames":[{"creatorName":"Hata, Shozaburo"}]},{"creatorNames":[{"creatorName":"Hatta, Mitsutoki"}]},{"creatorNames":[{"creatorName":"Kita, Tomo"}]},{"creatorNames":[{"creatorName":"Miyake, Yuki"}]},{"creatorNames":[{"creatorName":"Okamura, Kazuhiko"}]},{"creatorNames":[{"creatorName":"Tamaoki, Sachio"}]},{"creatorNames":[{"creatorName":"Ishikawa, Hiroyuki"}]},{"creatorNames":[{"creatorName":"Yamazaki, Jun"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-06"}],"displaytype":"detail","filename":"甲第299号_論文(石井太郎).pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第299号_論文(石井太郎)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/100/files/甲第299号_論文(石井太郎).pdf"},"version_id":"8217a2a6-b585-46a6-a9eb-67c47d5a35ff"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-06"}],"displaytype":"detail","filename":"甲第299号_論文内容の要旨(石井太郎).pdf","filesize":[{"value":"208.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第299号_論文内容の要旨(石井太郎)","url":"https://fdc.repo.nii.ac.jp/record/100/files/甲第299号_論文内容の要旨(石井太郎).pdf"},"version_id":"3b4e06ef-533c-449a-be98-a01a1b70dd67"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-06"}],"displaytype":"detail","filename":"甲第299号_論文審査結果の要旨(石井太郎).pdf","filesize":[{"value":"187.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第299号_論文審査結果の要旨(石井太郎)","url":"https://fdc.repo.nii.ac.jp/record/100/files/甲第299号_論文審査結果の要旨(石井太郎).pdf"},"version_id":"04fe3872-3c57-46a7-906d-39f0c6d036be"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Actins"},{"subitem_subject":"Animals"},{"subitem_subject":"Boron Compounds"},{"subitem_subject":"Cell Differentiation"},{"subitem_subject":"Cells, Cultured"},{"subitem_subject":"Epidermal Cells"},{"subitem_subject":"Epidermis"},{"subitem_subject":"Keratinocytes"},{"subitem_subject":"Myofibroblasts"},{"subitem_subject":"Primary Cell Culture"},{"subitem_subject":"RNA, Messenger"},{"subitem_subject":"RNA, Small Interfering"},{"subitem_subject":"Rats"},{"subitem_subject":"Rats, Wistar"},{"subitem_subject":"TRPV Cation Channels"},{"subitem_subject":"Transforming Growth Factor beta1"},{"subitem_subject":"Wound Healing"},{"subitem_subject":"transforming growth factor -β1"},{"subitem_subject":"TRPV2"},{"subitem_subject":"Actins","subitem_subject_language":"en"},{"subitem_subject":"Animals","subitem_subject_language":"en"},{"subitem_subject":"Boron Compounds","subitem_subject_language":"en"},{"subitem_subject":"Cell Differentiation","subitem_subject_language":"en"},{"subitem_subject":"Cells, Cultured","subitem_subject_language":"en"},{"subitem_subject":"Epidermal Cells","subitem_subject_language":"en"},{"subitem_subject":"Epidermis","subitem_subject_language":"en"},{"subitem_subject":"Keratinocytes","subitem_subject_language":"en"},{"subitem_subject":"Myofibroblasts","subitem_subject_language":"en"},{"subitem_subject":"Primary Cell Culture","subitem_subject_language":"en"},{"subitem_subject":"RNA, Messenger","subitem_subject_language":"en"},{"subitem_subject":"RNA, Small Interfering","subitem_subject_language":"en"},{"subitem_subject":"Rats","subitem_subject_language":"en"},{"subitem_subject":"Rats, Wistar","subitem_subject_language":"en"},{"subitem_subject":"TRPV Cation Channels","subitem_subject_language":"en"},{"subitem_subject":"Transforming Growth Factor beta1","subitem_subject_language":"en"},{"subitem_subject":"Wound Healing","subitem_subject_language":"en"},{"subitem_subject":"transforming growth factor -β1","subitem_subject_language":"en"},{"subitem_subject":"TRPV2","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"TRPV2 channel inhibitors attenuate fibroblast differentiation and contraction mediated by keratinocyte-derived TGF-β1 in an in vitro wound healing model of rats.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"TRPV2 channel inhibitors attenuate fibroblast differentiation and contraction mediated by keratinocyte-derived TGF-β1 in an in vitro wound healing model of rats.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["21"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-03-06"},"publish_date":"2019-03-06","publish_status":"0","recid":"100","relation_version_is_last":true,"title":["TRPV2 channel inhibitors attenuate fibroblast differentiation and contraction mediated by keratinocyte-derived TGF-β1 in an in vitro wound healing model of rats."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-06-25T22:32:53.709149+00:00"}