{"created":"2023-05-15T09:58:07.300662+00:00","id":10,"links":{},"metadata":{"_buckets":{"deposit":"19272921-f689-41c5-ae24-e79f0a83e06d"},"_deposit":{"created_by":15,"id":"10","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"10"},"status":"published"},"_oai":{"id":"oai:fdc.repo.nii.ac.jp:00000010","sets":["1:3:6"]},"author_link":[],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2014-03-15"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与大学","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"福岡歯科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"37114"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2013年度"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Elastic system fibers consist of microfibrils and tropoelastin. During development, microfibrils act as a template on which tropoelastin is deposited. Fibrillin-1 is the major component of microfibrils. It is not clear whether elastic fiber-associated molecules, such as fibulins, contribute to tropoelastin deposition. Among the fibulin family, fibulin-2, -4 and -5 are capable of binding to tropoelastin and fibrillin-1. In the present study, we used the RNA interference (RNAi) technique to establish individual gene-specific knockdown of fibulin-2, -4 and -5 in elastin-producing cells (human gingival fibroblasts; HGF). We then examined the extracellular deposition of tropoelastin using immunofluorescence. RNAi-mediated down-regulation of fibulin-4 and -5 was responsible for the diminution of tropoelastin deposition. Suppression of fibulin-5 appeared to inhibit the formation of fibrillin-1 microfibrils, while that of fibulin-4 did not. Similar results to those for HGF were obtained with human dermal fibroblasts. These results suggest that fibulin-4 and -5 may be associated in different ways with the extracellular deposition of tropoelastin during elastic fiber formation in elastin-producing cells in culture.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第254号"}]},"item_10006_relation_14":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Acta Histochem Cytochem. 2010 Dec 29;43(6):131-8. doi: 10.1267/ahc.10026. Epub 2010 Nov 20."}]}]},"item_10006_relation_15":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"J-STAGE"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/article/ahc/43/6/43_10026/_article","subitem_relation_type_select":"URI"}}]},"item_10006_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"著作権は日本組織細胞化学会が所有"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamauchi, Yoshinori"}]},{"creatorNames":[{"creatorName":"Tsuruga, Eichi"}]},{"creatorNames":[{"creatorName":"Nakashima, Kazuki"}]},{"creatorNames":[{"creatorName":"Sawa, Yoshihiko"}]},{"creatorNames":[{"creatorName":"Ishikawa, Hiroyuki"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-04-28"}],"displaytype":"detail","filename":"甲第254号_論文(山内).pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensefree":"著作権は日本組織細胞化学会が所有","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲第254号_論文(山内)","objectType":"fulltext","url":"https://fdc.repo.nii.ac.jp/record/10/files/甲第254号_論文(山内).pdf"},"version_id":"e92bcadf-2394-413d-8e2f-e39ea58695cf"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-04-28"}],"displaytype":"detail","filename":"甲第254号_論文内容の要旨(山内).pdf","filesize":[{"value":"71.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://fdc.repo.nii.ac.jp/record/10/files/甲第254号_論文内容の要旨(山内).pdf"},"version_id":"c9b62e15-0f6d-48f0-95b1-30d06ecfaf76"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-04-28"}],"displaytype":"detail","filename":"甲第254号_論文審査結果の要旨(山内).pdf","filesize":[{"value":"263.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"審査結果の要旨","url":"https://fdc.repo.nii.ac.jp/record/10/files/甲第254号_論文審査結果の要旨(山内).pdf"},"version_id":"36c439c2-8363-4878-8f14-8ff1e8577b06"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"トロポエラスチン"},{"subitem_subject":"Fibulin-4"},{"subitem_subject":"歯肉線維芽細胞"},{"subitem_subject":"弾性線維"},{"subitem_subject":"tropoelastin","subitem_subject_language":"en"},{"subitem_subject":"Fibulin-4","subitem_subject_language":"en"},{"subitem_subject":"human gingival fibroblasts","subitem_subject_language":"en"},{"subitem_subject":"elastic fiber","subitem_subject_language":"en"},{"subitem_subject":"Fibulin","subitem_subject_language":"en"},{"subitem_subject":"elastic fiber","subitem_subject_language":"en"},{"subitem_subject":"fibrillin","subitem_subject_language":"en"},{"subitem_subject":"microfibrils","subitem_subject_language":"en"},{"subitem_subject":"tropoelastin","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Fibulin-4 and -5, but not Fibulin-2, are Associated with Tropoelastin Deposition in Elastin-Producing Cell Culture.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Fibulin-4 and -5, but not Fibulin-2, are Associated with Tropoelastin Deposition in Elastin-Producing Cell Culture.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"15","path":["6"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-04-28"},"publish_date":"2014-04-28","publish_status":"0","recid":"10","relation_version_is_last":true,"title":["Fibulin-4 and -5, but not Fibulin-2, are Associated with Tropoelastin Deposition in Elastin-Producing Cell Culture."],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-06-24T01:54:51.896234+00:00"}