{"_buckets": {"deposit": "da0f8219-5179-4d29-9e74-3ac9d365f280"}, "_deposit": {"created_by": 15, "id": "56", "owners": [15], "pid": {"revision_id": 0, "type": "depid", "value": "56"}, "status": "published"}, "_oai": {"id": "oai:fdc.repo.nii.ac.jp:00000056", "sets": ["12"]}, "author_link": [], "item_10006_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2016-03-12"}]}, "item_10006_degree_grantor_9": {"attribute_name": "学位授与大学", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "福岡歯科大学"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "37114"}]}]}, "item_10006_degree_name_8": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(歯学) "}]}, "item_10006_description_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_description": "2015年度"}]}, "item_10006_description_7": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Osteoinductive scaffolds are required for bone tissue engineering. The aim of the present study was to assess the osteoinductive capacity of deoxyribonucleic acid (DNA)/protamine complexes in a rat model of critical-size calvarial defects. In addition, we investigated whether cultured mesenchymal-like cells (DP-cells) outgrown from DNA/protamine complex engrafted defects could differentiate to become osteogenic cells in vitro. DNA/protamine complexes were prepared by reactions between DNA and protamine sulfate solutions with stirring. Critical-sized (8mm) calvarial defects were created in the central parietal bones of adult rats. Defects were either left empty or treated with DNA/protamine complex scaffolds. Subsequently, micro-computed tomography (micro-CT), histological, and immunohistochemical analyses were performed. Micro-CT and histological assays showed that DNA/protamine complex engrafted defects had enhanced bone regeneration. DP-cells were expanded from explants of DNA/protamine complex engrafted defects using an explant outgrowth culture system. Osteogenesis-related factors were assessed in DP-cells after treatment with an osteoblast-inducing reagent (OIR). After 3months, nearly complete healing was observed for DNA/protamine complex engrafted calvarial defects. Increased alkaline phosphatase (ALP) activity and Alizarin red staining were found for cultured DP-cells. These cells had high expression levels of osteogenic genes, including those for RUNX-2, ALP, osteopontin, and osteocalcin. These results indicated that DNA/protamine complexes could facilitate bone regeneration in calvarial defects. Moreover, in vitro osteogenic induction experiments showed that DP-cells outgrown from DNA/protamine engrafted defects had an osteogenic potential. Based on these results, we suggest that DNA/protamine complexes may recruit osteocompetent cells in these defects, where they differentiate to osteogenic cells.", "subitem_description_type": "Abstract"}]}, "item_10006_dissertation_number_12": {"attribute_name": "報告番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲第275号"}]}, "item_10006_relation_14": {"attribute_name": "情報源", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "Bone. 2014 Oct;67:237-45. doi: 10.1016/j.bone.2014.07.018. Epub 2014 Jul 19."}]}]}, "item_10006_relation_15": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "ScienceDirect"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.sciencedirect.com/science/article/pii/S8756328214002701", "subitem_relation_type_select": "URI"}}]}, "item_10006_version_type_18": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Toda, Masako"}]}, {"creatorNames": [{"creatorName": "Ohno, Jun"}]}, {"creatorNames": [{"creatorName": "Shinozaki, Yosuke"}]}, {"creatorNames": [{"creatorName": "Ozaki, Masao"}]}, {"creatorNames": [{"creatorName": "Fukushima, Tadao"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-04-22"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "甲第275号_論文(戸田).pdf", "filesize": [{"value": "2.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 2700000.0, "url": {"label": "甲第275号_論文(戸田)", "objectType": "fulltext", "url": "https://fdc.repo.nii.ac.jp/record/56/files/甲第275号_論文(戸田).pdf"}, "version_id": "4e66ed92-0c49-4270-bcdf-c188884529fc"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-04-22"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "甲第275号_論文内容の要旨(戸田).pdf", "filesize": [{"value": "94.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 94300.0, "url": {"label": "要旨", "url": "https://fdc.repo.nii.ac.jp/record/56/files/甲第275号_論文内容の要旨(戸田).pdf"}, "version_id": "9757eaee-085c-4da5-a4ec-58e787e4ddaa"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-04-22"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "甲第275号_論文審査結果の要旨(戸田).pdf", "filesize": [{"value": "110.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 110000.0, "url": {"label": "審査結果の要旨", "url": "https://fdc.repo.nii.ac.jp/record/56/files/甲第275号_論文審査結果の要旨(戸田).pdf"}, "version_id": "2d7e6b23-49d6-4e0f-bfb6-dd846f08c3ff"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Animals", "subitem_subject_language": "en"}, {"subitem_subject": "Bone Regeneration", "subitem_subject_language": "en"}, {"subitem_subject": "DNA", "subitem_subject_language": "en"}, {"subitem_subject": "Male", "subitem_subject_language": "en"}, {"subitem_subject": "Osteogenesis", "subitem_subject_language": "en"}, {"subitem_subject": "Protamines", "subitem_subject_language": "en"}, {"subitem_subject": "Rats", "subitem_subject_language": "en"}, {"subitem_subject": "Rats, Sprague-Dawley", "subitem_subject_language": "en"}, {"subitem_subject": "Skull", "subitem_subject_language": "en"}, {"subitem_subject": "depression", "subitem_subject_language": "en"}, {"subitem_subject": "insomnia", "subitem_subject_language": "en"}, {"subitem_subject": "old-old", "subitem_subject_language": "en"}, {"subitem_subject": "oral-health related quality of life", "subitem_subject_language": "en"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Osteogenic potential for replacing cells in rat cranial defects implanted with a DNA/protamine complex paste.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Osteogenic potential for replacing cells in rat cranial defects implanted with a DNA/protamine complex paste.", "subitem_title_language": "en"}]}, "item_type_id": "10006", "owner": "15", "path": ["12"], "permalink_uri": "https://fdc.repo.nii.ac.jp/records/56", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2016-04-22"}, "publish_date": "2016-04-22", "publish_status": "0", "recid": "56", "relation": {}, "relation_version_is_last": true, "title": ["Osteogenic potential for replacing cells in rat cranial defects implanted with a DNA/protamine complex paste."], "weko_shared_id": -1}
Osteogenic potential for replacing cells in rat cranial defects implanted with a DNA/protamine complex paste.
https://fdc.repo.nii.ac.jp/records/56
https://fdc.repo.nii.ac.jp/records/56